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Literature summary extracted from

  • Wang, J.H.; Ma, W.C.; Su, J.C.; Chen, C.S.; Jiang, S.T.
    Comparison of the properties of m-calpain from Tilapia and grass shrimp muscles (1993), J. Agric. Food Chem., 41, 1379-1384.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.53 2-mercaptoethanol 1 mM, activates Penaeus monodon
3.4.22.53 2-mercaptoethanol 1 mM, activates Tilapia sp.
3.4.22.53 dithiothreitol 1 mM, activates Penaeus monodon
3.4.22.53 dithiothreitol 1 mM, activates Tilapia sp.
3.4.22.53 glutathione 1 mM, activates Penaeus monodon
3.4.22.53 glutathione 1 mM, activates Tilapia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 antipain 0.02 mM, 82% loss of activity Penaeus monodon
3.4.22.53 antipain 0.02 mM, 87% loss of activity Tilapia sp.
3.4.22.53 calpain inhibitor I 0.002 mM, 95% loss of activity Penaeus monodon
3.4.22.53 calpain inhibitor I 0.002 mM, 90% loss of activity Tilapia sp.
3.4.22.53 calpain inhibitor II 0.002 mM, 67% loss of activity Penaeus monodon
3.4.22.53 calpain inhibitor II 0.002 mM, 93% loss of activity Tilapia sp.
3.4.22.53 Cd2+ 5 mM Penaeus monodon
3.4.22.53 Cd2+ 5 mM Tilapia sp.
3.4.22.53 Co2+ 5 mM Penaeus monodon
3.4.22.53 Cu2+ 5 mM Penaeus monodon
3.4.22.53 Cu2+ 5 mM Tilapia sp.
3.4.22.53 Fe2+ 5 mM Penaeus monodon
3.4.22.53 Fe2+ 5 mM Tilapia sp.
3.4.22.53 Fe3+ 5 mM Penaeus monodon
3.4.22.53 Fe3+ 5 mM Tilapia sp.
3.4.22.53 Hg2+ 5 mM Penaeus monodon
3.4.22.53 Hg2+ 5 mM Tilapia sp.
3.4.22.53 iodoacetic acid 1 mM, complete loss of activity Penaeus monodon
3.4.22.53 iodoacetic acid 1 mM, 99% loss of activity Tilapia sp.
3.4.22.53 K+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
3.4.22.53 leupeptin 0.002 mM, complete loss of activity Penaeus monodon
3.4.22.53 leupeptin 0.002 mM, 93% loss of activity Tilapia sp.
3.4.22.53 Mg2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
3.4.22.53 Na+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
3.4.22.53 Ni2+ 5 mM Penaeus monodon
3.4.22.53 Ni2+ 5 mM Tilapia sp.
3.4.22.53 PCMB 1 mM, 56% inhibition Penaeus monodon
3.4.22.53 PCMB 1 mM, 42% inhibition Tilapia sp.
3.4.22.53 Zn2+ 5 mM Penaeus monodon
3.4.22.53 Zn2+ 5 mM Tilapia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ba2+ 5 mM, activates Penaeus monodon
3.4.22.53 Ba2+ 5 mM, activates Tilapia sp.
3.4.22.53 Ca2+ half-maximal activity at 2.4 mM, maximal activity at 5 mM Penaeus monodon
3.4.22.53 Ca2+ half-maximal activity at 0.23 mM, maximal activity at 1-2.5 mM Tilapia sp.
3.4.22.53 Co2+ 5 mM, activates Tilapia sp.
3.4.22.53 K+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
3.4.22.53 Mg2+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
3.4.22.53 Mn2+ 5 mM, activates Penaeus monodon
3.4.22.53 Mn2+ 5 mM, activates Tilapia sp.
3.4.22.53 Na+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
3.4.22.53 Sr2+ 5 mM, activates Penaeus monodon
3.4.22.53 Sr2+ 5 mM, activates Tilapia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.53 28000
-
 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.
3.4.22.53 80000
-
 2 * 80000, SDS-PAGE Penaeus monodon
3.4.22.53 80000
-
 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.
3.4.22.53 110000
-
 gel filtration Tilapia sp.
3.4.22.53 160000
-
 gel filtration Penaeus monodon

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Penaeus monodon
-
-
-
3.4.22.53 Tilapia sp.
-
 Tilapia nilotica * Tilapia aurea
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.53 muscle
-
 Penaeus monodon
-
3.4.22.53 muscle
-
 Tilapia sp.
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.53 -20°C, stable for 8 days, 70% inactivation after 2 months Penaeus monodon
3.4.22.53 -20°C, stable for 8 days, complete inactivation after 2 months Tilapia sp.

Subunits

EC Number Subunits Comment Organism
3.4.22.53 dimer 2 * 80000, SDS-PAGE Penaeus monodon
3.4.22.53 dimer 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.53 30
-
-
 Penaeus monodon
3.4.22.53 30
-
-
 Tilapia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.53 48.5
-
 10 min, 50% loss of activity Penaeus monodon
3.4.22.53 61
-
 10 min, 50% loss of activity Tilapia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.53 6.9
-
-
 Penaeus monodon
3.4.22.53 7.5
-
-
 Tilapia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.53 5 9 25°C, 30 min, stable in presence of 20 mM 2-mercaptoethanol Tilapia sp.
3.4.22.53 5.5
-
 25°C, 30 min, about 30% loss of activity Penaeus monodon
3.4.22.53 6.5 7.5 25°C, 30 min, stable in presence of 6 mM 2-mercaptoethanol Penaeus monodon
3.4.22.53 8.5
-
 25°C, 30 min, about 50% loss of activity Penaeus monodon