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Literature summary extracted from

  • Azarian, S.M.; Schlamp, C.L.; Williams, D.S.
    Characterization of calpain II in the retina and photoreceptor outer segments (1993), J. Cell Sci., 105, 787-798.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 calpeptin 50% inhibition of maximal caseinolytic activity at 10 nM and 21 nM for retinal and brain calpain Bos taurus
3.4.22.53 Ep-475 0.001 mM, 49% inhibition of the enzyme from retina, 46% inhibition of the enzyme from brain Bos taurus
3.4.22.53 leupeptin 0.001 mM, 67% inhibition of enzyme from retina and brain Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.53 cytoskeleton
-
 Bos taurus 5856
-
3.4.22.53 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ca2+ half maximal activity for retina and brain enzyme is 0.262 mM and 0.311 mM, maximal activity near 1 mM, no activity in presence of 0.03 mM Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.53 additional information Bos taurus the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.53
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.53 brain
-
 Bos taurus
-
3.4.22.53 photoreceptor outer segment Bos taurus
-
3.4.22.53 retina highest activity in plexiform layers and in the photoreceptor outer segments. In dark-adapted retinas the label is distributed throughout the outer segments. In light-adapted retinas, outer segment labelling is concentrated in the connecting cilium and the inner segments are labeled Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.53 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.53 alpha-actin + H2O slow degradation Bos taurus ?
-
 ?
3.4.22.53 casein + H2O
-
 Bos taurus ?
-
 ?
3.4.22.53 additional information no proteolysis of actin Bos taurus ?
-
 ?
3.4.22.53 additional information the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II Bos taurus ?
-
 ?
3.4.22.53 myosin II heavy chain + H2O rapid proteolysis Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.53 6.9
-
 enzyme from retina and brain Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.53 6 8 pH 6.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, enzyme from retina and brain Bos taurus