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Literature summary extracted from

  • Sargianos, N.; Gaitanaki, C.; Beis, I.
    Purification and characterization of m-calpain from the skeletal muscle of the amphibian Rana ridibunda (1994), J. Exp. Zool., 269, 95-105.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 antipain 0.01 mM 56% inhibition Pelophylax ridibundus
3.4.22.53 Cd2+ 5 mM, completely blocks activation of the enzyme by Ca2+ Pelophylax ridibundus
3.4.22.53 Co2+ 5 mM, completely blocks activation of the enzyme by Ca2+ Pelophylax ridibundus
3.4.22.53 Cu2+ 5 mM, completely blocks activation of the enzyme by Ca2+ Pelophylax ridibundus
3.4.22.53 E-64 0.01 mM 89% inhibition Pelophylax ridibundus
3.4.22.53 iodoacetamide 1 mM, 64% inhibition Pelophylax ridibundus
3.4.22.53 iodoacetic acid 1 mM, 76% inhibition Pelophylax ridibundus
3.4.22.53 leupeptin 0.01 mM 85% inhibition Pelophylax ridibundus
3.4.22.53 NEM 1 mM, 93% inhibition Pelophylax ridibundus
3.4.22.53 Ni2+ 5 mM, completely blocks activation of the enzyme by Ca2+ Pelophylax ridibundus
3.4.22.53 Zn2+ 5 mM, completely blocks activation of the enzyme by Ca2+ Pelophylax ridibundus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ba2+ 1 mM, decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ Pelophylax ridibundus
3.4.22.53 Ca2+ half-maximal activity at 0.4 mM, maximal activity at 1.5 mM Pelophylax ridibundus
3.4.22.53 Mg2+ 1 mM decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ Pelophylax ridibundus
3.4.22.53 Mn2+ 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM Pelophylax ridibundus
3.4.22.53 Sr2+ 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM. Synergistic activating effect with Ca2+ Pelophylax ridibundus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.53 28000
-
 1 * 78000 + 1 * 28000, SDS-PAGE Pelophylax ridibundus
3.4.22.53 78000
-
 1 * 78000 + 1 * 28000, SDS-PAGE Pelophylax ridibundus
3.4.22.53 105000
-
 gel filtration Pelophylax ridibundus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Pelophylax ridibundus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.53
-
 Pelophylax ridibundus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.53 skeletal muscle
-
 Pelophylax ridibundus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.53 additional information
-
-
 Pelophylax ridibundus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.53 casein + H2O
-
 Pelophylax ridibundus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.53 dimer 1 * 78000 + 1 * 28000, SDS-PAGE Pelophylax ridibundus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.53 25
-
-
 Pelophylax ridibundus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.53 55
-
 10 min, in absence of Ca2+, about 10% loss of activity Pelophylax ridibundus
3.4.22.53 60
-
 10 min, in absence of Ca2+, about 90% loss of activity Pelophylax ridibundus