EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.53 | antipain | 0.01 mM 56% inhibition | Pelophylax ridibundus | |
3.4.22.53 | Cd2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | Co2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | Cu2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | E-64 | 0.01 mM 89% inhibition | Pelophylax ridibundus | |
3.4.22.53 | iodoacetamide | 1 mM, 64% inhibition | Pelophylax ridibundus | |
3.4.22.53 | iodoacetic acid | 1 mM, 76% inhibition | Pelophylax ridibundus | |
3.4.22.53 | leupeptin | 0.01 mM 85% inhibition | Pelophylax ridibundus | |
3.4.22.53 | NEM | 1 mM, 93% inhibition | Pelophylax ridibundus | |
3.4.22.53 | Ni2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | Zn2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.53 | Ba2+ | 1 mM, decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | Ca2+ | half-maximal activity at 0.4 mM, maximal activity at 1.5 mM | Pelophylax ridibundus | |
3.4.22.53 | Mg2+ | 1 mM decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus | |
3.4.22.53 | Mn2+ | 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM | Pelophylax ridibundus | |
3.4.22.53 | Sr2+ | 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.53 | 28000 | - |
1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
3.4.22.53 | 78000 | - |
1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
3.4.22.53 | 105000 | - |
gel filtration | Pelophylax ridibundus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.53 | Pelophylax ridibundus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.53 | - |
Pelophylax ridibundus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.53 | skeletal muscle | - |
Pelophylax ridibundus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.22.53 | additional information | - |
- |
Pelophylax ridibundus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.53 | casein + H2O | - |
Pelophylax ridibundus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.53 | dimer | 1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.53 | 25 | - |
- |
Pelophylax ridibundus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.53 | 55 | - |
10 min, in absence of Ca2+, about 10% loss of activity | Pelophylax ridibundus |
3.4.22.53 | 60 | - |
10 min, in absence of Ca2+, about 90% loss of activity | Pelophylax ridibundus |