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Literature summary extracted from

  • Inomata, M.; Nomoto, M.; Hayashi, M.; Nakamura, M.; Imahori, K.; Kawashima, S.
    Comparison of low and high calcium requiring forms of the calcium-activated neutral protease (CANP) from rabbit skeletal muscle (1984), J. Biochem., 95, 1661-1670.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.52 antipain 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.52 E-64c 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.52 leupeptin 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.52 pepstatin A 1 mM, 60-80% inhibition Oryctolagus cuniculus
3.4.22.52 TLCK 0.1 mM, 60-80% inhibition Oryctolagus cuniculus
3.4.22.53 antipain 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.53 E-64c 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.53 leupeptin 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
3.4.22.53 pepstatin A 1 mM, 60-80% inhibition Oryctolagus cuniculus
3.4.22.53 TLCK 0.1 mM, 60-80% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.52 0.0081
-
 succinyl-casein pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.52 2
-
 succinyl-bovine serum albumin pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.52 63
-
 succinyl-protamine pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.52 283.5
-
 succinyl-insulin B pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.53 2.2
-
 succinyl-bovine serum albumin pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.53 10
-
 succinyl-casein pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.53 101.3
-
 succinyl-protamine pH 7.5, 25°C Oryctolagus cuniculus
3.4.22.53 453.7
-
 succinyl-insulin B pH 7.5, 25°C Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.52 Ba2+ activates, Ka: 1.5 mM Oryctolagus cuniculus
3.4.22.52 Ca2+ Ka-value : 0.05 mM Oryctolagus cuniculus
3.4.22.52 Mn2+ slight activation Oryctolagus cuniculus
3.4.22.52 Sr2+ activates, Ka: 0.45 mM Oryctolagus cuniculus
3.4.22.53 Ca2+ Ka-value: 0.7 mM Oryctolagus cuniculus
3.4.22.53 Sr2+ activates, Ka: 5.1 mM Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.52 Oryctolagus cuniculus
-
-
-
3.4.22.53 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.52 skeletal muscle
-
 Oryctolagus cuniculus
-
3.4.22.53 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.52 succinyl-bovine serum albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.52 succinyl-bovine-serum-albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.52 succinyl-casein + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.52 succinyl-insulin B + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.52 succinyl-protamine + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.53 succinyl-bovine serum albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.53 succinyl-bovine-serum-albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.53 succinyl-casein + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.53 succinyl-insulin B + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.53 succinyl-protamine + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.52 60
-
 10 min, 15% loss of activity Oryctolagus cuniculus
3.4.22.53 60
-
 10 min, complete loss of activity Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.52 7.3
-
-
 Oryctolagus cuniculus
3.4.22.53 7.6
-
-
 Oryctolagus cuniculus