Literature summary extracted from

Kitahara, A.; Sasaki, T.; Kikuchi, T.; Yumoto, N.; Yoshimura, N.; Hatanaka, M.; Murachi, T.
Large-scale purification of porcine calpain I and calpain II and comparison of proteolytic fragments of their subunits (1984), J. Biochem., 95, 1759-1766.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.52	calpastatin	-	Rattus norvegicus
3.4.22.52	calpastatin	-	Sus scrofa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.22.52	cytosol	-	Rattus norvegicus	5829	-
3.4.22.52	cytosol	-	Sus scrofa	5829	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.22.52	Ca2+	-	Rattus norvegicus
3.4.22.52	Ca2+	half-maximal activation at 0.0028 mM, maximal activation at 0.01 mM	Sus scrofa
3.4.22.53	Ca2+	half-maximal activation at 0.15 mM, maximal activation at 1 mM	Sus scrofa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.22.52	29000	-	1 * 83000 + 1 * 29000, SDS-PAGE	Sus scrofa
3.4.22.52	83000	-	1 * 83000 + 1 * 29000, SDS-PAGE	Sus scrofa
3.4.22.53	29000	-	1 * 80000 + 1 * 29000, SDS-PAGE	Sus scrofa
3.4.22.53	80000	-	1 * 80000 + 1 * 29000, SDS-PAGE	Sus scrofa

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.52	Rattus norvegicus	-	-	-
3.4.22.52	Sus scrofa	-	-	-
3.4.22.53	Rattus norvegicus	-	-	-
3.4.22.53	Sus scrofa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.52	large scale	Sus scrofa
3.4.22.53	large-scale	Sus scrofa

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.22.52	erythrocyte	-	Rattus norvegicus	-
3.4.22.52	erythrocyte	-	Sus scrofa	-
3.4.22.52	heart muscle	-	Rattus norvegicus	-
3.4.22.52	kidney	-	Rattus norvegicus	-
3.4.22.52	kidney	-	Sus scrofa	-
3.4.22.52	PK-15 cell	originated from kidney	Rattus norvegicus	-
3.4.22.52	skeletal muscle	-	Rattus norvegicus	-
3.4.22.52	spleen	-	Rattus norvegicus	-
3.4.22.52	submandibular gland	-	Rattus norvegicus	-
3.4.22.52	thymus	-	Rattus norvegicus	-
3.4.22.53	kidney	-	Rattus norvegicus	-
3.4.22.53	kidney	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.22.52	additional information	-	-	Rattus norvegicus
3.4.22.52	additional information	-	-	Sus scrofa
3.4.22.53	additional information	-	-	Rattus norvegicus
3.4.22.53	additional information	-	-	Sus scrofa

Subunits

EC Number	Subunits	Comment	Organism
3.4.22.52	dimer	-	Rattus norvegicus
3.4.22.52	dimer	1 * 83000 + 1 * 29000, SDS-PAGE	Sus scrofa
3.4.22.53	dimer	-	Rattus norvegicus
3.4.22.53	dimer	1 * 80000 + 1 * 29000, SDS-PAGE	Sus scrofa

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Release 2023.1 (January 2023)