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Literature summary extracted from

  • Dubin, G.; Chmiel, D.; Mak, P.; Rakwalska, M.; Rzychon, M.; Dubin, A.
    Molecular cloning and biochemical characterisation of proteases from Staphylococcus epidermidis (2001), Biol. Chem., 382, 1575-1582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.48 DNA and partial amino acid sequence determination and analysis Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.48 alpha2-Macroglobulin from human Staphylococcus epidermidis
3.4.22.48 E-64 strong Staphylococcus epidermidis
3.4.22.48 Hg2+ strong Staphylococcus epidermidis
3.4.22.48 iodoacetamide
-
 Staphylococcus epidermidis
3.4.22.48 additional information no inhibition by O-phenanthroline, diisofluorophosphate, phenylmethanesulfonyl fluoride, human kininogens and cystatins A,C, and D Staphylococcus epidermidis
3.4.22.48 Ni2+
-
 Staphylococcus epidermidis
3.4.22.48 p-hydroxymercuribenzoate
-
 Staphylococcus epidermidis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.48 extracellular
-
 Staphylococcus epidermidis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.48 19832
-
 x * 19832, DNA sequence determination Staphylococcus epidermidis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus epidermidis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.48 from culture supernatant, apparant homogenity Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.48 additional information
-
 purified enzyme, spectrophotometrical assay Staphylococcus epidermidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 alpha-1-antitrypsin + H2O limited proteolysis Staphylococcus epidermidis alpha-1-antitrypsin proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 HMW-kininogen + H2O limited proteolysis Staphylococcus epidermidis HMW-kininogen proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 protein + H2O
-
 Staphylococcus epidermidis peptide fragments
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.48 ? x * 19832, DNA sequence determination Staphylococcus epidermidis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 Ecp
-
 Staphylococcus epidermidis
3.4.22.48 extracellular cysteine protease
-
 Staphylococcus epidermidis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.48 37
-
 assay at Staphylococcus epidermidis