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Literature summary extracted from
- Salmonella enterica serovar Typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids (2000), J. Bacteriol., 182, 3383-3393.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.23 | divalent cations | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | KCl | stimulation, optimal at 50 mM | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | expression of a DNA library in Salmonella typhimurium, DNA and sequence determination and analysis, expression of the full length enzyme in Salmonella typhimurium strain TN5179 | Salmonella enterica |
| 3.4.11.23 | DNA sequence determination, overexpression from plasmid, Salmonella typhimurium strain TN5179 | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | K310V | increased specificty for Leu as Xaa, no activity with Asp-Leu in opposite to the wild-type enzyme | Salmonella enterica |
| 3.4.11.1 | R402W | reduced activity with Asp-Leu compared to the wild-type enzyme | Salmonella enterica |
| 3.4.11.23 | K310V | site-directed mutagenesis, more than 200fold reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.11.23 | R402W | site-directed mutagenesis, reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | bestatin | complete inhibition at 37° and 1 mM within 20 min | Salmonella enterica | |
| 3.4.11.1 | EDTA | inactivation can be restored by divalent cations | Salmonella enterica | |
| 3.4.11.23 | bestatin | complete inhibition at 1 mM, 37°C, after 20 min | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | EDTA | reversible by divalent cations | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 0.3 | - |
Asp-Phe | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 0.3 | - |
Asp-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 0.4 | - |
Asp-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 0.5 | - |
Asp-Leu-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 0.6 | - |
Asp-His | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 0.9 | - |
Glu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 1 | - |
Leu-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 1.8 | - |
Asp-Gln | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 3 | - |
Asp-Ile-Gly-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 4 | - |
Leu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 6 | - |
Asp-Leu-Lys | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.23 | additional information | - |
additional information | Km-values for diverse substrates, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 0.5 | - |
Asp-Leu-Gly | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 0.9 | - |
Glu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 4 | - |
Leu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | KCl | stimulates, optimal at 50 mM | Salmonella enterica | |
| 3.4.11.1 | Mg2+ | - |
Salmonella enterica | |
| 3.4.11.1 | additional information | enzyme is a metallopeptidase | Salmonella enterica |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 270000 | - |
gel filtration | Salmonella enterica |
| 3.4.11.23 | 45000 | - |
6 * 45000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.11.23 | 270000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | Ala-Leu + H2O | Salmonella enterica | - |
Ala + Leu | - |
? | |
| 3.4.11.1 | Asn-Leu + H2O | Salmonella enterica | - |
Asn + Leu | - |
? | |
| 3.4.11.1 | Asp-Leu + H2O | Salmonella enterica | - |
Asp + Leu | - |
? | |
| 3.4.11.1 | Cys-Leu + H2O | Salmonella enterica | - |
Cys + Leu | - |
? | |
| 3.4.11.1 | Glu-Leu + H2O | Salmonella enterica | - |
Glu + Leu | - |
? | |
| 3.4.11.1 | His-Leu + H2O | Salmonella enterica | - |
His + Leu | - |
? | |
| 3.4.11.1 | Leu-Leu + H2O | Salmonella enterica | - |
Leu + Leu | - |
? | |
| 3.4.11.1 | Met-Leu + H2O | Salmonella enterica | - |
Met + Leu | - |
? | |
| 3.4.11.1 | additional information | Salmonella enterica | no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL | ? | - |
? | |
| 3.4.11.1 | Ser-Leu + H2O | Salmonella enterica | - |
Ser + Leu | - |
? | |
| 3.4.11.1 | Thr-Leu + H2O | Salmonella enterica | - |
Thr + Leu | - |
? | |
| 3.4.11.23 | Ala-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Ala + Leu | - |
? | |
| 3.4.11.23 | Asn-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Asn + Leu | - |
? | |
| 3.4.11.23 | Asp-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | best substrate | Asp + Leu | - |
? | |
| 3.4.11.23 | Cys-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Cys + Leu | - |
? | |
| 3.4.11.23 | Glu-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Glu + Leu | - |
? | |
| 3.4.11.23 | His-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
His + Leu | - |
? | |
| 3.4.11.23 | Leu-Cys + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Cys | - |
? | |
| 3.4.11.23 | Leu-His + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + His | - |
? | |
| 3.4.11.23 | Leu-Ile + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Ile | - |
? | |
| 3.4.11.23 | Leu-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Leu | - |
? | |
| 3.4.11.23 | Leu-Met + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Met | - |
? | |
| 3.4.11.23 | Leu-Phe + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Phe | - |
? | |
| 3.4.11.23 | Leu-Tyr + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Tyr | - |
? | |
| 3.4.11.23 | Leu-Xaa + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa is an amino acid, substrate specificity, overview | Leu + Xaa | - |
? | |
| 3.4.11.23 | Leu-Xaa + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | lower activity compared to substrate Asp-Leu | Leu + Xaa | - |
? | |
| 3.4.11.23 | Met-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Met + Leu | - |
? | |
| 3.4.11.23 | Ser-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Ser + Leu | - |
? | |
| 3.4.11.23 | Thr-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Thr + Leu | - |
? | |
| 3.4.11.23 | Xaa-peptide + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | substrate specificity, overview | Xaa + peptide | - |
? | |
| 3.4.11.23 | Xaa-peptide + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa is an amino acid | Xaa + peptide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | Salmonella enterica | Q9RF52 | serovar typhimurium | - |
| 3.4.11.23 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene pepB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | recombinant enzyme from strain TN5179, 2.8fold | Salmonella enterica |
| 3.4.11.23 | recombinant from strain TN5179 | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.11.23 | release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln | residue K310 is involved in substrate specificity determination | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | additional information | - |
specific activity of the wild-type enzyme with Leu-Xaa, Asp-Xaa, Glu-Xaa and tripeptide substrates, overview | Salmonella enterica |
| 3.4.11.1 | 1 | - |
mutant K310V, substrate Asp-Leu | Salmonella enterica |
| 3.4.11.1 | 11 | - |
wild-type enzyme, substrate Ile-Leu and Val-Leu | Salmonella enterica |
| 3.4.11.1 | 13 | - |
wild-type enzyme, substrate Ser-Leu | Salmonella enterica |
| 3.4.11.1 | 18 | - |
wild-type enzyme, substrate Thr-Leu and Ala-Leu | Salmonella enterica |
| 3.4.11.1 | 25 | - |
wild-type enzyme, substrate Trp-Leu | Salmonella enterica |
| 3.4.11.1 | 32 | - |
wild-type enzyme, substrate Tyr-Leu | Salmonella enterica |
| 3.4.11.1 | 39 | - |
wild-type enzyme, substrate Phe-Leu | Salmonella enterica |
| 3.4.11.1 | 49 | - |
wild-type enzyme, substrate Gln-Leu | Salmonella enterica |
| 3.4.11.1 | 63 | - |
mutant R402W, substrate Asp-Leu | Salmonella enterica |
| 3.4.11.1 | 126 | - |
wild-type enzyme, substrate Asn-Leu | Salmonella enterica |
| 3.4.11.1 | 128 | - |
wild-type enzyme, substrate Cys-Leu | Salmonella enterica |
| 3.4.11.1 | 129 | 223 | purified recombinant wild-type enzyme, substrate Leu-Leu | Salmonella enterica |
| 3.4.11.1 | 137 | - |
mutant R402W, substrate Leu-Leu | Salmonella enterica |
| 3.4.11.1 | 156 | - |
wild-type enzyme, substrate His-Leu | Salmonella enterica |
| 3.4.11.1 | 164 | - |
wild-type enzyme, substrate Met-Leu | Salmonella enterica |
| 3.4.11.1 | 239 | - |
purified recombinant wild-type enzyme, substrate Asp-Leu | Salmonella enterica |
| 3.4.11.1 | 310 | - |
mutant K310V, substrate Leu-Leu | Salmonella enterica |
| 3.4.11.1 | 408 | - |
wild-type enzyme, substrate Glu-Leu | Salmonella enterica |
| 3.4.11.1 | 550 | - |
wild-type enzyme, substrate Asp-Leu | Salmonella enterica |
| 3.4.11.23 | additional information | - |
substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.11.23 | 190 | - |
purified recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | Ala-Leu + H2O | - |
Salmonella enterica | Ala + Leu | - |
? | |
| 3.4.11.1 | Asn-Leu + H2O | - |
Salmonella enterica | Asn + Leu | - |
? | |
| 3.4.11.1 | Asp-Gln + H2O | - |
Salmonella enterica | Asp + Gln | - |
? | |
| 3.4.11.1 | Asp-His + H2O | - |
Salmonella enterica | Asp + His | - |
? | |
| 3.4.11.1 | Asp-Ile-Gly-Gly + H2O | - |
Salmonella enterica | Asp + Ile-Gly-Gly | - |
? | |
| 3.4.11.1 | Asp-Leu + H2O | - |
Salmonella enterica | Asp + Leu | - |
? | |
| 3.4.11.1 | Asp-Leu-Gly + H2O | - |
Salmonella enterica | Asp + Leu-Gly | - |
? | |
| 3.4.11.1 | Asp-Leu-Lys + H2O | - |
Salmonella enterica | Asp + Leu-Lys | - |
? | |
| 3.4.11.1 | Asp-Phe + H2O | - |
Salmonella enterica | Asp + Phe | - |
? | |
| 3.4.11.1 | Asp-Tyr + H2O | - |
Salmonella enterica | Asp + Tyr | - |
? | |
| 3.4.11.1 | Cys-Leu + H2O | - |
Salmonella enterica | Cys + Leu | - |
? | |
| 3.4.11.1 | Gln-Leu + H2O | - |
Salmonella enterica | Gln + Leu | - |
? | |
| 3.4.11.1 | Glu-Leu + H2O | - |
Salmonella enterica | Glu + Leu | - |
? | |
| 3.4.11.1 | His-Leu + H2O | - |
Salmonella enterica | His + Leu | - |
? | |
| 3.4.11.1 | Ile-Leu + H2O | - |
Salmonella enterica | Ile + Leu | - |
? | |
| 3.4.11.1 | Leu-Leu + H2O | - |
Salmonella enterica | Leu + Leu | - |
? | |
| 3.4.11.1 | Leu-Tyr + H2O | - |
Salmonella enterica | Leu + Tyr | - |
? | |
| 3.4.11.1 | Met-Leu + H2O | - |
Salmonella enterica | Met + Leu | - |
? | |
| 3.4.11.1 | additional information | substrate specificity study, overview, Lys-Leu, Gly-Leu, Arg-Leu, and Pro-Leu are poor or no substrates | Salmonella enterica | ? | - |
? | |
| 3.4.11.1 | additional information | no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL | Salmonella enterica | ? | - |
? | |
| 3.4.11.1 | Phe-Leu + H2O | - |
Salmonella enterica | Phe + Leu | - |
? | |
| 3.4.11.1 | Ser-Leu + H2O | - |
Salmonella enterica | Ser + Leu | - |
? | |
| 3.4.11.1 | Thr-Leu + H2O | - |
Salmonella enterica | Thr + Leu | - |
? | |
| 3.4.11.1 | Trp-Leu + H2O | - |
Salmonella enterica | Trp + Leu | - |
? | |
| 3.4.11.1 | Tyr-Leu + H2O | - |
Salmonella enterica | Tyr + Leu | - |
? | |
| 3.4.11.1 | Val-Leu + H2O | - |
Salmonella enterica | Val + Leu | - |
? | |
| 3.4.11.23 | Ala-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Ala + Leu | - |
? | |
| 3.4.11.23 | Ala-Leu + H2O | 3% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ala + Leu | - |
? | |
| 3.4.11.23 | Asn-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Asn + Leu | - |
? | |
| 3.4.11.23 | Asn-Leu + H2O | 23% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asn + Leu | - |
? | |
| 3.4.11.23 | Asp-Ile + H2O | best substrate, 120% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Ile | - |
? | |
| 3.4.11.23 | Asp-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu | - |
? | |
| 3.4.11.23 | Asp-Leu + H2O | best substrate | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu | - |
? | |
| 3.4.11.23 | Asp-Leu-Gly + H2O | higher activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu-Gly | - |
? | |
| 3.4.11.23 | Asp-Phe + H2O | 105% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Phe | - |
? | |
| 3.4.11.23 | Asp-Xaa + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Xaa | - |
? | |
| 3.4.11.23 | Cys-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Cys + Leu | - |
? | |
| 3.4.11.23 | Cys-Leu + H2O | 23% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Cys + Leu | - |
? | |
| 3.4.11.23 | Gln-Leu + H2O | 9% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Gln + Leu | - |
? | |
| 3.4.11.23 | Glu-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Glu + Leu | - |
? | |
| 3.4.11.23 | Glu-Leu + H2O | 74% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Glu + Leu | - |
? | |
| 3.4.11.23 | His-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | His + Leu | - |
? | |
| 3.4.11.23 | His-Leu + H2O | 28% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | His + Leu | - |
? | |
| 3.4.11.23 | Ile-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ile + Leu | - |
? | |
| 3.4.11.23 | Leu-Cys + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Cys | - |
? | |
| 3.4.11.23 | Leu-Cys + H2O | 24% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Cys | - |
? | |
| 3.4.11.23 | Leu-His + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + His | - |
? | |
| 3.4.11.23 | Leu-His + H2O | 9% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + His | - |
? | |
| 3.4.11.23 | Leu-Ile + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Ile | - |
? | |
| 3.4.11.23 | Leu-Ile + H2O | 59% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Ile | - |
? | |
| 3.4.11.23 | Leu-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Leu | - |
? | |
| 3.4.11.23 | Leu-Leu + H2O | 40% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Leu | - |
? | |
| 3.4.11.23 | Leu-Met + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Met | - |
? | |
| 3.4.11.23 | Leu-Met + H2O | 30% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Met | - |
? | |
| 3.4.11.23 | Leu-Phe + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Phe | - |
? | |
| 3.4.11.23 | Leu-Phe + H2O | 12% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Phe | - |
? | |
| 3.4.11.23 | Leu-Tyr + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Tyr | - |
? | |
| 3.4.11.23 | Leu-Tyr + H2O | 37% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Tyr | - |
? | |
| 3.4.11.23 | Leu-Xaa + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Xaa | - |
? | |
| 3.4.11.23 | Leu-Xaa + H2O | lower activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Xaa | - |
? | |
| 3.4.11.23 | Met-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Met + Leu | - |
? | |
| 3.4.11.23 | Met-Leu + H2O | 30% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Met + Leu | - |
? | |
| 3.4.11.23 | Phe-Leu + H2O | 7% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Phe + Leu | - |
? | |
| 3.4.11.23 | Ser-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Ser + Leu | - |
? | |
| 3.4.11.23 | Ser-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ser + Leu | - |
? | |
| 3.4.11.23 | Thr-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Thr + Leu | - |
? | |
| 3.4.11.23 | Thr-Leu + H2O | 3% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Thr + Leu | - |
? | |
| 3.4.11.23 | Trp-Leu + H2O | 4% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Trp + Leu | - |
? | |
| 3.4.11.23 | Tyr-Leu + H2O | 6% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Tyr + Leu | - |
? | |
| 3.4.11.23 | Val-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Val + Leu | - |
? | |
| 3.4.11.23 | Xaa-Gly-Gly + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Gly-Gly | - |
? | |
| 3.4.11.23 | Xaa-Gly-Gly + H2O | low activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Gly-Gly | - |
? | |
| 3.4.11.23 | Xaa-Leu + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Leu | - |
? | |
| 3.4.11.23 | Xaa-peptide + H2O | substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + peptide | - |
? | |
| 3.4.11.23 | Xaa-peptide + H2O | Xaa is an amino acid | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + peptide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | hexamer | 6 * 46360-46362, mass spectrometry and DNA sequence calculation | Salmonella enterica |
| 3.4.11.23 | hexamer | 6 * 45000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.11.23 | hexamer | 6 * 46360-46362, mass spectrometry and amino acid sequence determination | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | LAP | - |
Salmonella enterica |
| 3.4.11.1 | PepB | - |
Salmonella enterica |
| 3.4.11.1 | peptidase B | - |
Salmonella enterica |
| 3.4.11.23 | PepB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 37 | - |
assay at | Salmonella enterica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 70 | - |
rapid loss of activity of crude cell extract, but the purified enzyme is stable | Salmonella enterica |
| 3.4.11.23 | 70 | - |
20 min, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 13 | - |
Asp-Leu-Lys | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 26 | - |
Asp-Ile-Gly-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 351 | - |
Leu-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 418 | - |
Asp-His | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 500 | - |
Asp-Phe | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 500 | - |
Asp-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 666 | - |
Asp-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 761 | - |
Asp-Gln | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 866 | - |
Glu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 918 | - |
Leu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.1 | 950 | - |
Asp-Leu-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
| 3.4.11.23 | additional information | - |
additional information | substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 866 | - |
Glu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 918 | - |
Leu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.4.11.23 | 950 | - |
Asp-Leu-Gly | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 8.5 | 9.5 | - |
Salmonella enterica |
| 3.4.11.23 | 8.5 | 9.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 7 | 10 | - |
Salmonella enterica |
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Release 2023.1 (January 2023)
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