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Literature summary extracted from

  • Gee, N.S.; Kenny, A.J.
    Proteins of the kidney microvillar membrane. Enzymic and molecular properties of aminopeptidase W (1987), Biochem. J., 246, 97-102.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.16 2-mercaptoethanol 50% inhibition at 0.6 mM Sus scrofa
3.4.11.16 amastatin 50% inhibition at 0.002 mM Sus scrofa
3.4.11.16 bestatin 50% inhibition at 0.006 mM Sus scrofa
3.4.11.16 captopril 50% inhibition at 0.3 mM Sus scrofa
3.4.11.16 Co2+ 65-90% inhibition at 1 mM Sus scrofa
3.4.11.16 Cu2+ 65-90% inhibition at 1 mM Sus scrofa
3.4.11.16 dithiothreitol 50% inhibition at 0.1 mM Sus scrofa
3.4.11.16 additional information many group-specific inhibitors for cysteine proteases have no effect Sus scrofa
3.4.11.16 Ni2+ 25% inhibition at 1 mM Sus scrofa
3.4.11.16 phosphate
-
 Sus scrofa
3.4.11.16 Zn2+ 65-90% inhibition at 1 mM, reactivated at 10 mM EDTA Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.16 0.488
-
 Leu-Trp
-
 Sus scrofa
3.4.11.16 0.568
-
 Glu-Trp
-
 Sus scrofa
3.4.11.16 0.625
-
 Ala-Trp
-
 Sus scrofa
3.4.11.16 1.7
-
 Pro-Trp
-
 Sus scrofa
3.4.11.16 1.757
-
 Gly-Trp
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.16 microvillus
-
 Sus scrofa 5902
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.16 Zn2+ 1.2 mol zinc per mol enzyme, probably involved in catalysis Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.16 Peptides + H2O Sus scrofa involved in intestinal digestion of small, pre-digested peptide fragments ?
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.16 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.16 glycoprotein 31% carbohydrates Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.16 intestine
-
 Sus scrofa
-
3.4.11.16 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.16 additional information
-
 activity highly buffer-dependent, optimal in 100 mM Tris-HCl Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.16 Ala-Trp + H2O
-
 Sus scrofa Ala + Trp
-
 ir
3.4.11.16 Arg-Trp + H2O
-
 Sus scrofa Arg + Trp
-
 ir
3.4.11.16 Asp-Phe + H2O
-
 Sus scrofa Asp + Phe
-
 ir
3.4.11.16 Glu-Trp + H2O
-
 Sus scrofa Glu + Trp
-
 ir
3.4.11.16 Glu-Tyr + H2O
-
 Sus scrofa Glu + Tyr
-
 ir
3.4.11.16 Gly-Trp + H2O
-
 Sus scrofa Gly + Trp
-
 ir
3.4.11.16 Gly-Tyr + H2O
-
 Sus scrofa Gly + Tyr
-
 ir
3.4.11.16 Ile-Phe + H2O
-
 Sus scrofa Ile + Phe
-
 ir
3.4.11.16 Ile-Trp + H2O
-
 Sus scrofa Ile + Trp
-
 ir
3.4.11.16 Leu-Phe + H2O
-
 Sus scrofa Leu + Phe
-
 ir
3.4.11.16 Leu-Trp + H2O
-
 Sus scrofa Leu + Trp
-
 ir
3.4.11.16 Leu-Tyr + H2O
-
 Sus scrofa Leu + Tyr
-
 ir
3.4.11.16 Peptides + H2O involved in intestinal digestion of small, pre-digested peptide fragments Sus scrofa ?
-
 ir
3.4.11.16 Phe-Phe + H2O
-
 Sus scrofa Phe + Phe
-
 ir
3.4.11.16 Phe-Trp + H2O
-
 Sus scrofa Phe + Trp
-
 ir
3.4.11.16 Phe-Tyr + H2O
-
 Sus scrofa Phe + Tyr
-
 ir
3.4.11.16 Pro-Trp + H2O
-
 Sus scrofa Pro + Trp
-
 ir
3.4.11.16 Trp-Phe + H2O
-
 Sus scrofa Trp + Phe
-
 ir
3.4.11.16 Trp-Trp + H2O
-
 Sus scrofa Trp + Trp
-
 ir
3.4.11.16 Tyr-Leu + H2O
-
 Sus scrofa Tyr + Leu
-
 ir
3.4.11.16 Tyr-Phe + H2O
-
 Sus scrofa Tyr + Phe
-
 ir
3.4.11.16 Val-Trp + H2O
-
 Sus scrofa Val + Trp
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.4.11.16 aminopeptidase W
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.16 29.6
-
 Pro-Trp
-
 Sus scrofa
3.4.11.16 38.5
-
 Ala-Trp
-
 Sus scrofa
3.4.11.16 92.6
-
 Leu-Trp
-
 Sus scrofa
3.4.11.16 113
-
 Glu-Trp
-
 Sus scrofa
3.4.11.16 127
-
 Gly-Trp
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.16 8
-
-
 Sus scrofa