BRENDA - Enzyme Database

D-Methylmalonyl-CoA hydrolase

Kovachy, R.J.; Stabler, S.P.; Allen, R.H.; Methods Enzymol. 166, 393-400 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.17
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.17
35000
-
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.17
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.17
1.41
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.17
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643854
Rattus norvegicus
methylmalonate + CoA
-
-
-
-
3.1.2.17
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
malonate + CoA
-
643854
Rattus norvegicus
-
3.1.2.17
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
?
-
-
-
-
3.1.2.17
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
propionate + CoA
-
643854
Rattus norvegicus
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.17
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.17
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.17
4
8.5
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
3.1.2.17
additional information
no cofactors identified
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
3.1.2.17
additional information
no cofactors identified
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.17
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.17
35000
-
-
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.17
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.17
1.41
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.17
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643854
Rattus norvegicus
methylmalonate + CoA
-
-
-
-
3.1.2.17
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
malonate + CoA
-
643854
Rattus norvegicus
-
3.1.2.17
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
?
-
-
-
-
3.1.2.17
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643854
Rattus norvegicus
propionate + CoA
-
643854
Rattus norvegicus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.17
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.17
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.17
4
8.5
-
Rattus norvegicus