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Literature summary extracted from

  • Kovachy, R.J.; Stabler, S.P.; Allen, R.H.
    D-Methylmalonyl-CoA hydrolase (1988), Methods Enzymol., 166, 393-400.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.17 0.7
-
 (S)-methylmalonyl-CoA pH 6.0, 30°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.17 35000
-
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.17 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.17
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.17 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.17 1.41
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.17 -20°C, purified enzyme stable for at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.17 (S)-methylmalonyl-CoA + H2O not active on the L-isomer Rattus norvegicus methylmalonate + CoA
-
 ?
3.1.2.17 malonyl-CoA + H2O 16% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus malonate + CoA
-
 ?
3.1.2.17 additional information acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus ?
-
 ?
3.1.2.17 propionyl-CoA + H2O 3% of the activity with (S)-methylmalonyl-CoA Rattus norvegicus propionate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.17 monomer 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.2.17 D-methylmalonyl-CoA hydrolase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.17 23.3
-
 (S)-methylmalonyl-CoA pH 6.0, 30°C Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.17 4 8.5
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.2.17 additional information no cofactors identified Rattus norvegicus