BRENDA - Enzyme Database

Recognition, isolation, and characterization of rat liver D-methylmalonyl coenzyme A hydrolase

Kovachy, R.J.; Copley, S.D.; Allen, R.H.; J. Biol. Chem. 258, 11415-11421 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.17
acetyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
butyryl-CoA
competitive
Rattus norvegicus
3.1.2.17
CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
decanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
glutaryl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
heptanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
hexanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
malonyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
nonanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
octanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
palmitoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
pentanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
propionyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
succinyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
tridecanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.17
0.7
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.4
-
DL-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.9
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
14
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.17
35000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
Rattus norvegicus
-
methylmalonate + CoA
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.17
partially
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.17
1.41
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.17
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
-
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
malonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
?
-
-
-
-
3.1.2.17
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
propionate + CoA
-
643853
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.17
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.17
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.17
6
-
50% activity at pH 5.3 and pH 7.1
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.17
0.01
-
palmitoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.04
-
tridecanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.05
-
decanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.2
-
nonanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.5
-
octanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.6
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.4
-
CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.5
-
heptanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.5
-
succinyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.7
-
hexanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
2.6
-
butyryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
2.6
-
valeryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
3
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
4.1
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
4.9
-
glutaryl-CoA
pH 6.0, 30°C
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.17
acetyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
butyryl-CoA
competitive
Rattus norvegicus
3.1.2.17
CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
decanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
glutaryl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
heptanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
hexanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
malonyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
nonanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
octanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
palmitoyl-CoA
noncompetitive inhibition
Rattus norvegicus
3.1.2.17
pentanoyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
propionyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
succinyl-CoA
competitive inhibition
Rattus norvegicus
3.1.2.17
tridecanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.17
0.01
-
palmitoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.04
-
tridecanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.05
-
decanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.2
-
nonanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.5
-
octanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.6
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.4
-
CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.5
-
heptanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.5
-
succinyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.7
-
hexanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
2.6
-
butyryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
2.6
-
valeryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
3
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
4.1
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
4.9
-
glutaryl-CoA
pH 6.0, 30°C
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.17
0.7
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.4
-
DL-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
1.9
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
3.1.2.17
14
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.17
35000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
Rattus norvegicus
-
methylmalonate + CoA
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.17
partially
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.17
1.41
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.17
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.17
(S)-methylmalonyl-CoA + H2O
-
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
malonate + CoA
-
643853
Rattus norvegicus
?
3.1.2.17
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
?
-
-
-
-
3.1.2.17
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
propionate + CoA
-
643853
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.17
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.17
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.17
6
-
50% activity at pH 5.3 and pH 7.1
Rattus norvegicus