EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.17 | acetyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | butyryl-CoA | competitive | Rattus norvegicus | |
3.1.2.17 | CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | decanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
3.1.2.17 | glutaryl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | heptanoyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | hexanoyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | malonyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | nonanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
3.1.2.17 | octanoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
3.1.2.17 | palmitoyl-CoA | noncompetitive inhibition | Rattus norvegicus | |
3.1.2.17 | pentanoyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | propionyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | succinyl-CoA | competitive inhibition | Rattus norvegicus | |
3.1.2.17 | tridecanoyl-CoA | noncompetitive inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.17 | 0.7 | - |
malonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.7 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.4 | - |
DL-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.9 | - |
propionyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 14 | - |
acetyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.17 | 35000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.17 | (S)-methylmalonyl-CoA + H2O | Rattus norvegicus | - |
methylmalonate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.17 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.17 | partially | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.2.17 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.2.17 | 1.41 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.2.17 | -20°C, purified enzyme stable for at least 1 month | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.17 | (S)-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | methylmalonate + CoA | - |
? | |
3.1.2.17 | (S)-methylmalonyl-CoA + H2O | not active on the L-isomer | Rattus norvegicus | methylmalonate + CoA | - |
? | |
3.1.2.17 | acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
3.1.2.17 | DL-methylmalonyl-CoA + H2O | - |
Rattus norvegicus | CoA + methylmalonate | - |
? | |
3.1.2.17 | malonyl-CoA + H2O | 16% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | malonate + CoA | - |
? | |
3.1.2.17 | additional information | acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? | |
3.1.2.17 | propionyl-CoA + H2O | 3% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | propionate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.17 | monomer | 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.17 | 23.3 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.17 | 6 | - |
50% activity at pH 5.3 and pH 7.1 | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.17 | 0.01 | - |
palmitoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.04 | - |
tridecanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.05 | - |
decanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.2 | - |
nonanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.5 | - |
octanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 0.6 | - |
malonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.4 | - |
CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.5 | - |
succinyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.5 | - |
heptanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 1.7 | - |
hexanoyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 2.6 | - |
butyryl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 2.6 | - |
valeryl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 3 | - |
propionyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 4.1 | - |
acetyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
3.1.2.17 | 4.9 | - |
glutaryl-CoA | pH 6.0, 30°C | Rattus norvegicus |