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Literature summary extracted from

  • Sramek, S.J.; Frerman, F.E.
    Purification and properties of Escherichia coli coenzyme A-transferase (1975), Arch. Biochem. Biophys., 171, 14-26.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.9 epsilon-Acetyl-CoA
-
 Escherichia coli
2.8.3.9 additional information partially inactivated by acyl-CoA substrates in absence of carboxylic acid substrates Escherichia coli
2.8.3.9 p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.9 0.33
-
 acetyl-CoA pH 8.1, 24°C Escherichia coli
2.8.3.9 6.7
-
 epsilon-Acetyl-CoA pH 8.1, 24°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.9 membrane membrane-associated Escherichia coli 16020
-
2.8.3.9 spheroplast
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.9 23000
-
 2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE Escherichia coli
2.8.3.9 26000
-
 2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE Escherichia coli
2.8.3.9 97800
-
 sedimentation equilibrium centrifugation Escherichia coli
2.8.3.9 98000
-
 SDS-PAGE Escherichia coli
2.8.3.9 99000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.9 butanoate + acetoacetyl-CoA Escherichia coli
-
 butanoyl-CoA + acetoacetate
-
 r
2.8.3.9 butanoate + acetoacetyl-CoA Escherichia coli C22
-
 butanoyl-CoA + acetoacetate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.9 Escherichia coli
-
 C22
-
2.8.3.9 Escherichia coli C22
-
 C22
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.3.9 additional information contains no detectable carbohydrate Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.9
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.9 117
-
 fraction B Escherichia coli
2.8.3.9 160
-
 fraction A Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.9 4-pentenoate + acetoacetyl-CoA reaction at 21% the rate of acetate Escherichia coli 4-pentenoyl-CoA + acetoacetate
-
 r
2.8.3.9 acetate + acetoacetyl-CoA best substrates Escherichia coli acetyl-CoA + acetoacetate
-
 r
2.8.3.9 acetate + acetoacetyl-CoA best substrates Escherichia coli C22 acetyl-CoA + acetoacetate
-
 r
2.8.3.9 acetyl-CoA + acetoacetate best substrates Escherichia coli acetate + acetoacetyl-CoA
-
 r
2.8.3.9 acetyl-CoA + acetoacetate best substrates Escherichia coli C22 acetate + acetoacetyl-CoA
-
 r
2.8.3.9 butanoate + acetoacetyl-CoA
-
 Escherichia coli butanoyl-CoA + acetoacetate
-
 r
2.8.3.9 butanoate + acetoacetyl-CoA reaction at 68% the rate of acetate Escherichia coli butanoyl-CoA + acetoacetate
-
 r
2.8.3.9 butanoate + acetoacetyl-CoA
-
 Escherichia coli C22 butanoyl-CoA + acetoacetate
-
 r
2.8.3.9 butanoate + acetoacetyl-CoA reaction at 68% the rate of acetate Escherichia coli C22 butanoyl-CoA + acetoacetate
-
 r
2.8.3.9 butyrate + acetyl-CoA reaction at 56% the rate of acetoacetate, reverse reaction at 86% the rate of acetoacetyl-CoA Escherichia coli butyryl-CoA + acetate
-
 r
2.8.3.9 crotonyl-CoA + acetate reaction at 4% the rate of acetoacetylCoA Escherichia coli crotonate + acetyl-CoA
-
 r
2.8.3.9 DL-3-hydroxybutyrate + acetyl-CoA reaction at 9% the rate of acetoacetate Escherichia coli 3-hydroxybutyryl-CoA + acetate
-
 r
2.8.3.9 epsilon-acetyl-CoA + acetoacetate reaction at 1% the rate of acetylCoA Escherichia coli acetate + acetoacetyl-CoA
-
 r
2.8.3.9 formate + acetoacetyl-CoA reaction at 7% the rate of acetate Escherichia coli formyl-CoA + acetoacetate
-
 r
2.8.3.9 additional information decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates Escherichia coli ?
-
 ?
2.8.3.9 additional information decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates Escherichia coli C22 ?
-
 ?
2.8.3.9 octanoate + acetoacetyl-CoA reaction at 3% the rate of acetate Escherichia coli octanoyl-CoA + acetoacetate
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.3.9 tetramer 2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.9 10.6
-
 epsilon-Acetyl-CoA pH 8.1, 24°C Escherichia coli