BRENDA - Enzyme Database

Submitochondrial localization and partial purification of the succinylCoA: 3-hydroxy-3-methylglutarate coenzyme A transferase from rat liver

Deana, R.; Rigoni, F.; Donella Deana, A.; Galzigna, L.; Biochim. Biophys. Acta 662, 119-124 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.3.13
2-mercaptoethanol
increases activity
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.8.3.13
addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes
Rattus norvegicus
2.8.3.13
mercaptoethanol improves stability
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.13
acetoacetate
non-competitive inhibition
Rattus norvegicus
2.8.3.13
malonate
competitive inhibition
Rattus norvegicus
2.8.3.13
succinate
competitive inhibition of the product
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.13
0.22
-
succinyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
2.8.3.13
0.37
-
malonyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
2.8.3.13
1.7
-
3-hydroxy-3-methylglutarate
pH 8.5, 30°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.3.13
mitochondrion
mainly in the mitochondrial matrix, also in the inner membrane
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.13
42000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.3.13
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
-
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.3.13
Rattus norvegicus
-
Wistar albino
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.3.13
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.13
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.13
0.0194
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.8.3.13
-20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.13
butyryl-CoA + 3-hydroxy-3-methylglutarate
only negligible amounts of HMGCoA produced
643832
Rattus norvegicus
butyrate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
?
2.8.3.13
malonyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
malonate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
2.8.3.13
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.3.13
8.5
-
-
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.8.3.13
6
-
decreasing the pH to lower values causes an irreversible loss of the enzyme activity
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.13
0.5
-
acetoacetate
pH 8.5, 30°C
Rattus norvegicus
2.8.3.13
1.5
-
succinate
pH 8.5, 30°C
Rattus norvegicus
2.8.3.13
2.1
-
malonate
pH 8.5, 30°C
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.3.13
2-mercaptoethanol
increases activity
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.8.3.13
addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes
Rattus norvegicus
2.8.3.13
mercaptoethanol improves stability
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.13
acetoacetate
non-competitive inhibition
Rattus norvegicus
2.8.3.13
malonate
competitive inhibition
Rattus norvegicus
2.8.3.13
succinate
competitive inhibition of the product
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.13
0.5
-
acetoacetate
pH 8.5, 30°C
Rattus norvegicus
2.8.3.13
1.5
-
succinate
pH 8.5, 30°C
Rattus norvegicus
2.8.3.13
2.1
-
malonate
pH 8.5, 30°C
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.13
0.22
-
succinyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
2.8.3.13
0.37
-
malonyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
2.8.3.13
1.7
-
3-hydroxy-3-methylglutarate
pH 8.5, 30°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.3.13
mitochondrion
mainly in the mitochondrial matrix, also in the inner membrane
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.13
42000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.3.13
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
-
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.3.13
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.13
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.13
0.0194
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.3.13
-20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.13
butyryl-CoA + 3-hydroxy-3-methylglutarate
only negligible amounts of HMGCoA produced
643832
Rattus norvegicus
butyrate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
?
2.8.3.13
malonyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
malonate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
2.8.3.13
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.3.13
8.5
-
-
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.8.3.13
6
-
decreasing the pH to lower values causes an irreversible loss of the enzyme activity
Rattus norvegicus