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Literature summary extracted from

  • Dimroth, P.; Loyal, R.; Eggerer, H.
    Characterization of the isolated transferase subunit of citrate lyase as a CoA-transferase. Evidence against a covalent enzyme-substrate intermediate (1977), Eur. J. Biochem., 80, 479-488.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.10 iodoacetate
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.10 0.2
-
 (3S)-citryl-CoA pH 8.0, 25°C Klebsiella aerogenes
2.8.3.10 1.3
-
 acetyl-CoA pH 8.0, 25°C, isolated transferase Klebsiella aerogenes
2.8.3.10 1.3
-
 butyryl-CoA pH 8.0, 25°C, isolated transferase Klebsiella aerogenes
2.8.3.10 1.3
-
 propionyl-CoA pH 8.0, 25°C, isolated transferase Klebsiella aerogenes
2.8.3.10 1.3
-
 acetyl-dephospho-CoA pH 8.0, 25°C, isolated transferase Klebsiella aerogenes
2.8.3.10 3.3
-
 propionyl-CoA pH 8.0, 25°C, integrated into deacetyl citrate lyase Klebsiella aerogenes
2.8.3.10 6.7
-
 acetyl-CoA pH 8.0, 25°C, integrated into deacetyl citrate lyase Klebsiella aerogenes
2.8.3.10 12.5
-
 acetyl-dephospho-CoA pH 8.0, 25°C, integrated into deacetyl citrate lyase Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.10 acetyl-CoA + citrate Klebsiella aerogenes biologically significant reaction of transferase subunit as part of citrate lyase, the isolated transferase subunit represents not only an acetyl-thioacyl carrier protein, citrate acyl carrier protein transferase but also an acetyl-CoA:citrate CoA-transferase acetate + (3S)-citryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.10 Klebsiella aerogenes
-
 ATCC 13882
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.10 complete EC 4.1.3.6 citrate lyase with 3 subunits, EC 2.8.3.10 is the largest subunit Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.10 9.1
-
 acetyl-CoA, integrated into enzyme complex Klebsiella aerogenes
2.8.3.10 25
-
 acetyl-CoA, isolated transferase subunit Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.8.3.10 -70°C, stable Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.10 acetyl-CoA + citrate
-
 Klebsiella aerogenes acetate + (3S)-citryl-CoA
-
 r
2.8.3.10 acetyl-CoA + citrate biologically significant reaction of transferase subunit as part of citrate lyase, the isolated transferase subunit represents not only an acetyl-thioacyl carrier protein, citrate acyl carrier protein transferase but also an acetyl-CoA:citrate CoA-transferase Klebsiella aerogenes acetate + (3S)-citryl-CoA
-
 r
2.8.3.10 acetyl-dephospho-CoA + citrate
-
 Klebsiella aerogenes acetate + (3S)-citryl-dephospho-CoA
-
 r
2.8.3.10 acetyl-[acyl-carrier protein] + citrate
-
 Klebsiella aerogenes acetate + (3S)-citryl-[acyl-carrier protein]
-
 r
2.8.3.10 butyryl-CoA + citrate
-
 Klebsiella aerogenes butyrate + (3S)-citryl-CoA
-
 r
2.8.3.10 additional information also catalyzes a citrate independent exchange reaction of acetyl residues between acetyl-thioacyl carrier protein or acetyl-CoA and acetate Klebsiella aerogenes ?
-
 ?
2.8.3.10 propionyl-CoA + citrate
-
 Klebsiella aerogenes propionate + (3S)-citryl-CoA
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.10 21.7
-
 acetyl-CoA pH 8.0, 25°C Klebsiella aerogenes
2.8.3.10 26.7
-
 (3S)-citryl-CoA pH 8.0, 25°C Klebsiella aerogenes