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Literature summary extracted from

  • Allen, A.K.; Rosenberg, H.
    The mechanism of action and some properties of serine ethanolamine phosphate synthetase (1968), Biochim. Biophys. Acta, 151, 504-519.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.8.4 stable to freezing, aggregates on thawing Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.4 Ca2+
-
 Gallus gallus
2.7.8.4 CDP strong inhibition Gallus gallus
2.7.8.4 CMP competitive inhibition to CDPethanolamine, non-competitive inhibition to L-serine Gallus gallus
2.7.8.4 iodoacetamide 1 mM, 6% remaining activity Gallus gallus
2.7.8.4 iodoacetate 1 mM, 42% remaining activity Gallus gallus
2.7.8.4 L-alanine 27-34% inhibition of the reaction Gallus gallus
2.7.8.4 L-homoserine 27-34% inhibition of the reaction Gallus gallus
2.7.8.4 L-threonine 27-34% inhibition of the reaction Gallus gallus
2.7.8.4 Methylmercuric bromide 0.1 mM, 6% remaining activity Gallus gallus
2.7.8.4 Methylmercuric iodide 0.1 mM, 3% remaining activity Gallus gallus
2.7.8.4 additional information iodoacetate is less effective as inhibitor, DFP is no inhibitor Gallus gallus
2.7.8.4 N-Acetylimidazole 10 mM, 8% remaining activity Gallus gallus
2.7.8.4 N-ethylmaleimide 0.2 mM, 25% remaining activity Gallus gallus
2.7.8.4 p-hydroxymercuribenzoate 0.1 mM, 2% remaining activity Gallus gallus
2.7.8.4 phenylmercuric acetate 0.1 mM, 2% remaining activity Gallus gallus
2.7.8.4 Sulfanilic acid diazotized, 0.2 mM, 36% remaining activity Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.4 0.011
-
 CMP-aminoethylphosphonate pH 7.0, 37°C Gallus gallus
2.7.8.4 0.085
-
 CDP-ethanolamine pH 7.0, 37°C Gallus gallus
2.7.8.4 1
-
 L-serine pH 7.0, 37°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.4 microsome
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.4 Co2+
-
 Gallus gallus
2.7.8.4 Mg2+ bivalent cations are essential for activity, Mg2+ is most effective Gallus gallus
2.7.8.4 Mn2+
-
 Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.4 CDP-ethanolamine + L-serine Gallus gallus
-
 CMP + L-serine-ethanolamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.4 Gallus gallus
-
 chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.4 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.4 gut
-
 Gallus gallus
-
2.7.8.4 intestinal mucosa
-
 Gallus gallus
-
2.7.8.4 kidney
-
 Gallus gallus
-
2.7.8.4 additional information no activity in liver, spleen, brain, oviduct, blood, and heart Gallus gallus
-
2.7.8.4 pancreas
-
 Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
2.7.8.4 0°C, stable to freezing Gallus gallus
2.7.8.4 4°C, enzyme preparartion remains active Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.4 CDP-2-amino-2-methylpropanol + L-serine
-
 Gallus gallus ?
-
 ?
2.7.8.4 CDP-ethanolamine + DL-alpha-methylserine
-
 Gallus gallus ?
-
 ?
2.7.8.4 CDP-ethanolamine + L-serine
-
 Gallus gallus CMP + L-serine-ethanolamine phosphate
-
 ?
2.7.8.4 CDP-ethanolamine + L-serine absolutely specific for serine, both stereoisomers are active Gallus gallus CMP + L-serine-ethanolamine phosphate
-
 ?
2.7.8.4 CMP-aminoethylphosphonate + L-serine
-
 Gallus gallus ?
-
 ?
2.7.8.4 additional information CDP-choline and CDP-serine cannot replace CDP-ethanolamine, N-acetyl-L-serine, L-homoserine, 4-hydroxy-L-proline, 5-hydroxy-DL-lysine, 3-hydroxy-DL-glutamic acid, L-threonine, ethanolamine or 3-hydroxypropionic acid cannot replace serine Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.4 SEP synthetase
-
 Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.4 37
-
 stable for 60 min Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.4 6.5 9 55% of maximal activity at pH 6.5, 30% of maximal activity at pH 9.0 Gallus gallus