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Literature summary extracted from

  • Hopper, J.E.; Dickinson, D.B.
    Sugar nucleotide inhibition of UDP-glucose pyrophosphorylase from calf liver (1973), Biochim. Biophys. Acta, 309, 307-317.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 alpha-D-galactose 1-phosphate no inhibition Bos taurus
2.7.7.9 additional information inhibition kinetics for UDP-sugars; little or no inhibition by alpha-D-mannose 1-phosphate, alpha-D-xylose 1-phosphate or alpha-D-glucuronic acid 1-phosphate Bos taurus
2.7.7.9 UDP-D-mannose
-
 Bos taurus
2.7.7.9 UDP-galactose 14% inhibition at 0.35 mM Bos taurus
2.7.7.9 UDP-galacturonic acid 48% inhibition at 0.43 m Bos taurus
2.7.7.9 UDP-glucose 87% inhibition at 0.08 mM Bos taurus
2.7.7.9 UDP-glucuronic acid 56% inhibition at 0.45 mM Bos taurus
2.7.7.9 UDP-mannose 23% inhibition at 0.45 mM Bos taurus
2.7.7.9 UDP-xylose 32% inhibition at 0.36 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.03 0.048 alpha-D-glucose 1-phosphate pH 7.5, 30°C Bos taurus
2.7.7.9 0.1 0.2 UTP pH 7.5, 30°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ activation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Bos taurus
-
 male calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 8.95
-
 partially purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Bos taurus diphosphate + UDP-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 7.5
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.9 0.005
-
 UDP-D-glucose pH 7.5, 30°C Bos taurus
2.7.7.9 0.15
-
 UDP-D-glucuronic acid pH 7.5, 30°C Bos taurus
2.7.7.9 0.21
-
 UDP-D-galacturonic acid pH 7.5, 30°C Bos taurus
2.7.7.9 0.24
-
 UDP-D-xylose pH 7.5, 30°C Bos taurus
2.7.7.9 4.8
-
 UDP-D-galactose pH 7.5, 30°C Bos taurus
2.7.7.9 9.6
-
 UDP-D-mannose pH 7.5, 30°C Bos taurus