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Literature summary extracted from

  • Brishammar, S.; Juntti, N.
    RNA-synthesizing enzymes in healthy and TMV-infected tobacco leaves. Partial purification and characterization to tobacco polynucleotide phosphorylase (1974), Arch. Biochem. Biophys., 164, 224-232.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.8 Bis-(3-aminopropyl)-amine optimal polymerization activity requires presence, 2fold increase in activity at 30 mM Nicotiana tabacum
2.7.7.8 dithiothreitol similar activation as with 2-mercaptoethanol Nicotiana tabacum
2.7.7.8 mercaptoethanol 20 mM, 3fold activation Nicotiana tabacum
2.7.7.8 NH4+ maximal activation at 100 mM Nicotiana tabacum
2.7.7.8 putrescine optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM Nicotiana tabacum
2.7.7.8 spermidine optimal polymerization activity requires polyamines, 1.4fold increase in activity at 30 mM Nicotiana tabacum
2.7.7.8 spermine optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM Nicotiana tabacum

General Stability

EC Number General Stability Organism
2.7.7.8 insensitive to freezing Nicotiana tabacum
2.7.7.8 stable against several freezing and thawing cycles Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 additional information polymerization reaction is not inhibited by diphosphate Nicotiana tabacum
2.7.7.8 phosphate 0.1 mM, complete inhibition of polymerization activity Nicotiana tabacum
2.7.7.8 Poly(A) inhibition of poly(U) synthesis Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 K+ maximal activation at 200 mm Nicotiana tabacum
2.7.7.8 Mg2+ Mg2+ or Mn2+ required for activity, maximal activation at 1 mM Mg2+, inhibition above Nicotiana tabacum
2.7.7.8 Mn2+ Mg2+ or Mn2+ required for activity, maximal activation at 0.06 mM Mn2+, inhibition above Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 150000
-
 sedimentation analysis Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Nicotiana tabacum
-
 L. var Samsun, healthy and TMV-infected leaves
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 DEAE-Sephadex, enzyme from healthy and TMV-infected leaves Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.8 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 0.0002
-
 incorporation of UDP into polynucleotide Nicotiana tabacum

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 -75°C, several months, no loss of activity Nicotiana tabacum
2.7.7.8 4°C, 1 week, 50% loss of activity Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Nicotiana tabacum poly(A)+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate synthetic activity enhanced in presence of a primer Nicotiana tabacum RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Nicotiana tabacum RNAn+1 + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 30
-
 60 min assay Nicotiana tabacum
2.7.7.8 35
-
 10 min assay Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8.5
-
 polymerization of ADP Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.8 7.6 8.8 approx. 40% of maximal activity at pH 7.6, approx. 70% of maximal activity at pH 8.8 Nicotiana tabacum