Literature summary extracted from

Nolden, W.T.; Richter, G.
polynucleotide phosphorylase from a cyanobacterium (Synechococcus sp.): subunit composition and properties (1982), Z. Naturforsch. C, 37c, 600-608.

No PubMed abstract available

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.7.8	KCl	maximal activation at 300 mM	Synechococcus sp.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.7.8	Na2HPO4	0.0032 mM, complete inhibition	Synechococcus sp.
2.7.7.8	sucrose	50%, approx. 50% inhibition of partially purified enzyme	Synechococcus sp.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.7.8	K+	-	Synechococcus sp.
2.7.7.8	Mg2+	preferentially activated by Mg2+	Synechococcus sp.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.7.8	70000	-	alpha4, 4 * 70000, SDS-PAGE	Synechococcus sp.
2.7.7.8	275000	-	native PAGE	Synechococcus sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.8	Synechococcus sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.8	ammonium sulfate, DEAE-cellulose, gel filtration, poly(A)-Sepharose	Synechococcus sp.

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.7.8	0.2	-	-	Synechococcus sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.8	RNAn + a nucleoside diphosphate	-	Synechococcus sp.	RNAn+1 + phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.7.8	tetramer	alpha4, 4 * 70000, SDS-PAGE	Synechococcus sp.

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.7.8	37	-	assay at	Synechococcus sp.

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
2.7.7.8	Synechococcus sp.	isoelectric focusing	-	4.2

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Release 2023.1 (January 2023)