Literature summary extracted from

Ochoa, S.; Mii, S.
Enzymatic synthesis of polynucleotides. IV. Purification and properties of polynucleotide phosphorylase of Azobacter vinelandii (1961), J. Biol. Chem., 236, 3303-3311.

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.8	Azotobacter vinelandii	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.8	ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite	Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.7.8	39.6	-	-	Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.8	poly(G) + GDP	much lower activity than with ADP, activity depends on polyribonucleotide primer	Azotobacter vinelandii	poly(G)+1 + phosphate	-	?
2.7.7.8	ribonucleoside 5'-diphosphate + phosphate	-	Azotobacter vinelandii	ribonucleoside 5'-diphosphate + phosphate	exchange reaction	?
2.7.7.8	RNAn + a nucleoside diphosphate	synthesis of poly(A): no primer addition required if large amounts of enzyme or Mg2+ are used, with small amounts of either component a primer is required, poly(G) synthesis: primer required	Azotobacter vinelandii	RNAn+1 + phosphate	-	?

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Release 2023.1 (January 2023)