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Literature summary extracted from

  • Takagi, M.; Nishioka, M.; Kakihara, H.; Kitabayashi, M.; Inoue, H.; Kawakami, B.; Oka, M.; Imanaka, T.
    Characterization of DNA polymerase from sp. strain KOD1 and its application to PCR (1997), Appl. Environ. Microbiol., 63, 4504-4510.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.7 biotechnology thermostable polymerase used in PCR Thermococcus kodakarensis
2.7.7.7 molecular biology thermostable polymerase used in PCR Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7
-
 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Thermococcus kodakarensis
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Thermococcus kodakarensis P77933
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Thermococcus kodakarensis diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 75
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 95
-
 50% loss of activity after 12 h Thermococcus kodakarensis
2.7.7.7 100
-
 50% loss of activity after 3 h Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 6.5
-
-
 Thermococcus kodakarensis