EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.7.7 | biotechnology | thermostable polymerase used in PCR | Thermus thermophilus |
2.7.7.7 | molecular biology | thermostable polymerase used in PCR | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.7 | dimethyl sulfoxide | 67% inhibition of RNAse H activity at 10% | Thermus thermophilus | |
2.7.7.7 | glycerol | 33% inhibition of RNAse H activity at 10% | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.7 | Mg2+ | enzyme prefers Mn2+ over Mg2+ for RNase H activity | Thermus thermophilus | |
2.7.7.7 | Mn2+ | enzyme prefers Mn2+ over Mg2+ for RNase H activity, optimal concentration: 1.5-2.5 mM | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | deoxynucleoside triphosphate + DNAn | Thermus thermophilus | - |
diphosphate + DNAn+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | deoxynucleoside triphosphate + DNAn | - |
Thermus thermophilus | diphosphate + DNAn+1 | - |
? | |
2.7.7.7 | deoxynucleoside triphosphate + DNAn | enzyme also has RNAse H/exonuclease 5'--3' activity, enzyme prefers RNA/DNA substrate over DNA/DNA duplex | Thermus thermophilus | diphosphate + DNAn+1 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.7 | 70 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.7 | 70 | 75 | optimal RNAse H activity at 70°C, sharp decline of activity above 75°C | Thermus thermophilus |