Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fansler, B.S.; Loeb, L.A.
    Sea urchin nuclear DNA polymerase (1974), Methods Enzymol., 29, 53-70.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.7 sucrose, dextrose and glycerol stabilize Strongylocentrotus purpuratus
2.7.7.7 sucrose, dextrose and glycerol stabilize Mesocentrotus franciscanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 1,10-phenanthroline
-
 Mesocentrotus franciscanus
2.7.7.7 ethanol 3%, 50% inhibition Mesocentrotus franciscanus
2.7.7.7 Sodium dodecyl sulfate
-
 Mesocentrotus franciscanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Strongylocentrotus purpuratus
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Mesocentrotus franciscanus
2.7.7.7 Mg2+ stimulates at 9 mM Mesocentrotus franciscanus
2.7.7.7 Mn2+ stimulates at 0.5 mM, about a third the maximal activity with Mg2+ Mesocentrotus franciscanus
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Strongylocentrotus purpuratus
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Mesocentrotus franciscanus
2.7.7.7 Zn2+ 10-13% of the activity with Mg2+, optimal concentration: 0.3-0.5 mM Mesocentrotus franciscanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 150000 168000 gel filtration, glycerol gradient centrifugation Mesocentrotus franciscanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Strongylocentrotus purpuratus
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Mesocentrotus franciscanus
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Mesocentrotus franciscanus
-
 sea urchin
-
2.7.7.7 Strongylocentrotus purpuratus
-
 sea urchin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Mesocentrotus franciscanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.7 embryo
-
 Strongylocentrotus purpuratus
-
2.7.7.7 embryo
-
 Mesocentrotus franciscanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 0.027
-
-
 Strongylocentrotus purpuratus
2.7.7.7 0.027
-
-
 Mesocentrotus franciscanus

Storage Stability

EC Number Storage Stability Organism
2.7.7.7 -70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years Strongylocentrotus purpuratus
2.7.7.7 -70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years Mesocentrotus franciscanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Strongylocentrotus purpuratus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Mesocentrotus franciscanus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity Mesocentrotus franciscanus diphosphate + DNAn+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.8 8.2
-
 Strongylocentrotus purpuratus
2.7.7.7 7.8 8.2
-
 Mesocentrotus franciscanus