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Literature summary extracted from

  • Boyer, A.S.; Hallick, R.B.
    Purification and characterization of a soluble DNA-dependent chloroplast RNA polymerase from Pisum sativum (1998), Plant Sci., 137, 13-32.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 (NH4)2SO4
-
 Pisum sativum
2.7.7.6 heparin
-
 Pisum sativum
2.7.7.6 Tagetitoxin 50% inhibition at 0.0001 mM. Complete inhibition at 0.01 mM Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.6 0.1
-
 GTP pH 7.9, 23°C Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.6 chloroplast
-
 Pisum sativum 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 KCl maximal activity at 2 mM KCl Pisum sativum
2.7.7.6 Mg2+ maximal activity at 15 mM MgCl2 or MnCl2 Pisum sativum
2.7.7.6 Mn2+ maximal activity at 15 mM MgCl2 or MnCl2 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 35000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 42000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 51000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 54000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 75000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 81000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 84000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 95000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 110000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 120000
-
 x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum
2.7.7.6 620000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.6 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.006
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 GTP + RNAn
-
 Pisum sativum diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Pisum sativum diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 40
-
-
 Pisum sativum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.6 30 50 30°C: about 50% of maximal activity, 50°C: about 20% of maximal activity Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.6 7 8.7 pH 7.0: about 75% of maximal activity, pH 8.7: about 75% of maximal activity Pisum sativum