Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ding, H.F.; Winkler, H.H.
    Purification and partial characterization of the DNA-dependent RNA polymerase from Rickettsia prowazekii (1990), J. Bacteriol., 172, 5624-5630.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.6 membrane 74% of the RNA polymerase activity is associated with the rickettsial cell membrane at a high salt concentration, 600 mM NaCl Rickettsia prowazekii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ required. Optimal activity at 10 mM MgCl2 and 50 mM NaCl Rickettsia prowazekii
2.7.7.6 NaCl optimal activity at 50 mM NaCl2 Rickettsia prowazekii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 34500
-
 betax,beta‘x,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE Rickettsia prowazekii
2.7.7.6 85000
-
 betax,beta‘x,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE Rickettsia prowazekii
2.7.7.6 142000
-
 betax,beta‘x,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE Rickettsia prowazekii
2.7.7.6 148000
-
 betax,beta‘x,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE Rickettsia prowazekii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Rickettsia prowazekii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Rickettsia prowazekii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.0168
-
-
 Rickettsia prowazekii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Rickettsia prowazekii diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? betax,beta‘x,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE Rickettsia prowazekii