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Literature summary extracted from

  • Fujita, M.; Amemura, A.
    Purification and characterization of a DNA-dependent RNA polymerase from Pseudomonas putida (1992), Biosci. Biotechnol. Biochem., 56, 1797-1800.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 rifampicin 0.00006 mg/ml, 50% inactivation Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ maximal activity in presence of 5-10 mM MgCl2 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 42000
-
 betax,beta‘x,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE Pseudomonas putida
2.7.7.6 87000
-
 betax,beta‘x,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE Pseudomonas putida
2.7.7.6 151000
-
 betax,beta‘x,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE Pseudomonas putida
2.7.7.6 156000
-
 betax,beta‘x,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Pseudomonas putida
-
 PpY101
-
2.7.7.6 Pseudomonas putida PpY101
-
 PpY101
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Pseudomonas putida diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Pseudomonas putida PpY101 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? betax,beta‘x,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.6 37 50 37°C: about 50% of maximal activity, 50°C: about 80% of maximal activity Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.6 30
-
 10 min, stable Pseudomonas putida
2.7.7.6 40
-
 10 min, slight inactivation Pseudomonas putida
2.7.7.6 50
-
 10 min, slight inactivation Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 8
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.6 7 9 pH 7.0: about 30% of maximal activity, pH 9.0: about 60% of maximal activity Pseudomonas putida