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Literature summary extracted from

  • Wnendt, S.; Hartmann, R.K.; Ulbrich, N.; Erdmann, V.A.
    Isolation and physical properties of the DNA-directed RNA polymerase from Thermus thermophilus HB8 (1990), Eur. J. Biochem., 191, 467-472.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.6 spermidine optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine Thermus thermophilus
2.7.7.6 thermine optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 MnCl2 in presence of 10 mM MgCl2 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 KCl optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine Thermus thermophilus
2.7.7.6 Mg2+ optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 40000
-
 betax,beta‘x,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE Thermus thermophilus
2.7.7.6 92000
-
 betax,beta‘x,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE Thermus thermophilus
2.7.7.6 140000
-
 betax,beta‘x,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE Thermus thermophilus
2.7.7.6 170000
-
 betax,beta‘x,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Thermus thermophilus
-
 HB8
-
2.7.7.6 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.03
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn calf thymus DNA as template Thermus thermophilus diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Thermus thermophilus diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn calf thymus DNA as template Thermus thermophilus HB8 / ATCC 27634 / DSM 579 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Thermus thermophilus HB8 / ATCC 27634 / DSM 579 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? betax,beta‘x,alpha,x,sigmax, x * 140000 + x * 170000 + x * 40000 + x * 92000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 65
-
-
 Thermus thermophilus