Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cudny, H.; Deutscher, M.P.
    3 Processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement (1988), J. Biol. Chem., 263, 1518-1523.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.56 KCl enzyme works optimally at 50 mM KCl, inhibition at 200 mM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.56 Co2+ no effect Escherichia coli
2.7.7.56 KCl enzyme works optimally at 50 mM KCl, inhibition at 200 mM Escherichia coli
2.7.7.56 Mg2+ required Escherichia coli
2.7.7.56 Mg2+ optimal activity at 3 mM Escherichia coli
2.7.7.56 Mn2+ no effect Escherichia coli
2.7.7.56 additional information no effect: Cd2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.56 tRNAn+1 + phosphate Escherichia coli brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate tRNA + a nucleoside diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.56 Escherichia coli
-
 strain deficient in 5 ribonucleases
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.56 poly(A) + phosphate
-
 Escherichia coli ?
-
 r
2.7.7.56 tRNAn+1 + phosphate
-
 Escherichia coli tRNA + a nucleoside diphosphate
-
 r
2.7.7.56 tRNAn+1 + phosphate brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate Escherichia coli tRNA + a nucleoside diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.56 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.56 8 9
-
 Escherichia coli