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Literature summary extracted from

  • Taube, R.; Loya, S.; Avidan, O.; Perach, M.; Hizi, A.
    Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization (1998), Biochem. J., 329, 579-587.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.49 construction of a plasmid that induces in bacteria the synthesis of an enzymatically active reverse transcriptase, expression of a protein with a six-histidine tag in Escherichia coli mouse mammary tumor virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.49 1,10-phenanthroline
-
mouse mammary tumor virus
2.7.7.49 2',3'-dideoxyguanosine triphosphate
-
mouse mammary tumor virus
2.7.7.49 2',3'-dideoxythymidine 5'-triphosphate
-
mouse mammary tumor virus
2.7.7.49 3'-Azido-2',3'-dideoxythymidine 5'-triphosphate
-
mouse mammary tumor virus
2.7.7.49 KCl IC50: 40-50 mM mouse mammary tumor virus
2.7.7.49 NaCl IC50: 40-50 mM mouse mammary tumor virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.49 additional information
-
additional information Km-value for poly(rA)n*oligo(dT)12-18 is 0.004 mg/ml, Km-value for poly(rC)n*oligo(dG)12-18 is 0.0036 mg/ml, measured at pH 7.5 and 37°C mouse mammary tumor virus
2.7.7.49 0.0034
-
dGTP pH 7.5, 37°C mouse mammary tumor virus
2.7.7.49 0.0045
-
dTTP pH 7.5, 37°C mouse mammary tumor virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.49 Mg2+ marked preference for Mg2+ over Mn2+ mouse mammary tumor virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.49 66000
-
glycerol gradient ultracentrifugation mouse mammary tumor virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 mouse mammary tumor virus
-
BR6 milk-transmitted strain
-
2.7.7.49 mouse mammary tumor virus BR6
-
BR6 milk-transmitted strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.49 recombinant enzyme with a six-histidine tag mouse mammary tumor virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
mouse mammary tumor virus diphosphate + DNAn+1
-
?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
mouse mammary tumor virus BR6 diphosphate + DNAn+1
-
?
2.7.7.49 dGTP + DNAn
-
mouse mammary tumor virus diphosphate + DNAn+1
-
?
2.7.7.49 dGTP + DNAn
-
mouse mammary tumor virus BR6 diphosphate + DNAn+1
-
?
2.7.7.49 dTTP + poly(rA)/(dT)18
-
mouse mammary tumor virus ?
-
?
2.7.7.49 dTTP + poly(rA)/(dT)18
-
mouse mammary tumor virus BR6 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.49 monomer 1 * 66000 mouse mammary tumor virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.49 7.5
-
-
mouse mammary tumor virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.49 7 10 pH 7.0: about 90% of maximal activity, pH 10.0: about 50% of maximal activity mouse mammary tumor virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.49 0.0085
-
3'-Azido-2',3'-dideoxythymidine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus
2.7.7.49 0.034
-
2',3'-dideoxythymidine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus
2.7.7.49 0.054
-
2',3'-dideoxyguanosine 5'-triphosphate pH 7.5, 37°C mouse mammary tumor virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.49 40 50 IC50: 40-50 mM mouse mammary tumor virus NaCl
2.7.7.49 40 50 IC50: 40-50 mM mouse mammary tumor virus KCl