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Literature summary extracted from

  • Carroll, S.S.; Sardana, V.; Yang, Z.; Jacobs, A.R.; Mizenko, C.; Hall, D.; Hill, L.; Zugay-Murphy, J.; Kuo, L.C.
    Only a small fraction of purified hepatitis C RNA-dependent RNA polymerase is catalytically competent: implications for viral replication and in vitro assays (2000), Biochemistry, 39, 8243-8249.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.0006
-
 CTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.0006
-
 UTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.0014
-
 GTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.0029
-
 ATP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.0057
-
 UTP pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA) Hepacivirus C
2.7.7.48 0.013
-
 UTP pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA) Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
-
 expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 C-terminally truncated enzyme form NS5B(DELTA21) expressed in Escherichia coli Hepacivirus C

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.48 0.025
-
 truncated enzyme form NS5B(DELTA21) Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 ATP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 CTP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 GTP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn once synthesis has begun, the C-terminally truncated enzyme NS5B(DELTA21) does not dissociate from the template until a complete double strand copy of the RNA is made Hepacivirus C diphosphate + RNAn+1
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.48 0.000105
-
 ATP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.000133
-
 CTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.000167
-
 UTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.000383
-
 GTP pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21) Hepacivirus C
2.7.7.48 0.00167
-
 UTP pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA) Hepacivirus C
2.7.7.48 0.0272
-
 UTP pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA) Hepacivirus C