Literature summary extracted from

Yamashita, T.; Kaneko, S.; Shirota, Y.; Qin, W.; Nomura, T.; Kobayashi, K.; Murakami, S.
RNA-dependent RNA polymerase activity of the soluble recombinant Hepatitis C virus NS5B protein truncated at the C-terminal region (1998), J. Biol. Chem., 273, 15479-15486.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.48	soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt which lacks the C-terminal 21 amino acid residues, expressed in Escherichia coli	Hepacivirus C

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.7.7.48	additional information	soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt which lacks the C-terminal 21 amino acid residues, expressed in Escherichia coli. The recombinant soluble enzyme exhibits RNA-dependent RNA polymerase activity in vitro which is dependent on template and primer, but it does not exhibit the terminal transferase activity	Hepacivirus C

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.7.48	additional information	the C-termimnal region of NS5B, which is dispensable for the RNA-dependent RNA polymerase activity, dramatically affects the subcellular localization of NS5B retaining it in perinuclear sites in transiently overexpressed mammalian cells. The C-terminal region of NS5B, especially the putative anchoring domain has a role in retaining the protein in the cytoplasm	Hepacivirus C	-	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.48	Hepacivirus C	-	expressed in Escherichia coli	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.48	soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt, expressed in Escherichia coli	Hepacivirus C

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.7.48	0.00035	-	-	Hepacivirus C

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.48	nucleoside triphosphate + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?

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Release 2023.1 (January 2023)