Literature summary extracted from
Alaoui-Lsmaili, M.H.; Hamel, M.; L'Heureux, L.; Nicolas, O.; Bilimoria, D.; Labonte, P.; Mounir, S.; Rando, R.F.
The Hepatitis C virus NS5B RNA-dependent RNA polymerase activity and susceptibility to inhibitors is modulated by metal cations (2000), J. Hum. Virol., 3, 306-316.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.48 |
recombinant full-length enzyme is expressed in insect cells |
Hepacivirus C |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
2',3'-dideoxycytidine triphosphate |
inhibition is higher in presence of Mn2+ than in presence of Mg2+ |
Hepacivirus C |
|
2.7.7.48 |
2',3'-dideoxyguanosine triphosphate |
inhibition is higher in presence of Mn2+ than in presence of Mg2+ |
Hepacivirus C |
|
2.7.7.48 |
2'-deoxycytidine triphosphate |
inhibition is higher in presence of Mn2+ than in presence of Mg2+ |
Hepacivirus C |
|
2.7.7.48 |
2'-dGTP |
inhibition is higher in presence of Mn2+ than in presence of Mg2+ |
Hepacivirus C |
|
2.7.7.48 |
gliotoxin |
in presence of MnCl2 the IC50 value is 0.036 mM, in presence of Mn2+ the IC50 value is 0.202 mM |
Hepacivirus C |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
additional information |
- |
additional information |
effect of Mg2+ and Mn2+ on the Km-value for polyC/oligoG and polyA/oligodT |
Hepacivirus C |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
Mg2+ |
optimal polymerase activity at 10 mM MgCl2 |
Hepacivirus C |
|
2.7.7.48 |
Mn2+ |
optimal polymerase activity at 0.25 mM MnCl2, activity is lower than in presence of MgCl2 |
Hepacivirus C |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Hepacivirus C |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.48 |
recombinant full-length enzyme expressed in baculovirus Sf9 insect cells |
Hepacivirus C |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
polyC/oligoG is more efficient in supporting the HCV NS5B polymerase activity than polyA/oligodT. PolyA/oligoU or polyI/oligodC |
Hepacivirus C |
diphosphate + RNAn+1 |
- |
? |
|
IC50 Value
EC Number |
IC50 Value |
IC50 Value Maximum |
Comment |
Organism |
Inhibitor |
Structure |
---|
2.7.7.48 |
0.202 |
- |
in presence of MnCl2 the IC50 value is 0.036 mM, in presence of Mn2+ the IC50 value is 0.202 mM |
Hepacivirus C |
gliotoxin |
|