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Literature summary extracted from

  • Donofrio, J.C.; Kuchta, J.; Moore, R.; Kaczmarczyk, W.
    Properties of a solubilized replicase isolated from corn infected with Maize dwarf mosaic virus (1986), Can. J. Microbiol., 32, 637-644.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.48 RNA 2-10fold stimulation with the addition of various RNA templates. CCMV and CPMV RNA produces the greatest stimulation. TMV RNA also acts as a template, but with much less efficiency Maize dwarf mosaic virus
2.7.7.48 Sodium diethyldithiocarbamate 5 mM, great stimulation of template-dependent activity and to a lesser extent template-independent activity Maize dwarf mosaic virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 Mg2+ 12.5 mM, replicase activity is reduced by 50% Maize dwarf mosaic virus
2.7.7.48 RNase completely eliminates incorporation Maize dwarf mosaic virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.48 additional information the enzyme is associated with particulate fraction of Zea mays leaf infected with Maize dwarf mosaic virus Maize dwarf mosaic virus
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-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ maximal activity at 2.8 mM Maize dwarf mosaic virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 160000
-
 glycerol density gradient sedimentation Maize dwarf mosaic virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Maize dwarf mosaic virus
-
 Zea mays infected with
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.48 additional information leaf of Zea mays infected with Maize dwarf mosaic virus Maize dwarf mosaic virus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Maize dwarf mosaic virus diphosphate + RNAn+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 7.7
-
-
 Maize dwarf mosaic virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.48 6.3 9.7 pH 6.3: about 45% of maximal activity, pH 9.7: about 35% of maximal activity Maize dwarf mosaic virus