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Literature summary extracted from

  • Chu, P.W.G.; Westaway, E.G.
    Characterization of Kunjin virus RNA-dependent RNA polymerase: reinitiation of synthesis in vitro (1987), Virology, 157, 330-337.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.48 after one cycle of freezing at -70°C and thawing, the activity is about 90% of the unfrozen control, but is reduced to 40-50% after two cycles Kunjin virus
2.7.7.48 enzyme is crude extract is very stable Kunjin virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ optimal activity at 10-12 mM Kunjin virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Kunjin virus
-
 Vero cells infected with
-
2.7.7.48 Kunjin virus MRM61C
-
 Vero cells infected with
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.48 4°C, storage for 2-3 d after a freeze-thaw cycle, stable Kunjin virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn incorporation is more dependent on exogenopus UTP and GTP than ATP or CTP Kunjin virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn incorporation is more dependent on exogenopus UTP and GTP than ATP or CTP Kunjin virus MRM61C diphosphate + RNAn+1
-
 ?