Literature summary extracted from
Clark, G.L.; Peden, K.W.C.; Symons, R.H.
Cucumber mosaic virus-induced RNA polymerase: partial purification and properties of the template-free enzyme (1974), Virology, 62, 434-444.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
pancreatic RNase |
0.006 mg/ml, marked inhibition |
Cucumber mosaic virus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
Mg2+ |
optimal concentration is 13 mM |
Cucumber mosaic virus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Cucumber mosaic virus |
- |
cucumber cotyledons infected with |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.48 |
partial |
Cucumber mosaic virus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.7.7.48 |
0.00088 |
- |
reaction with CMV RNA |
Cucumber mosaic virus |
2.7.7.48 |
0.0015 |
- |
reaction with poly(C) |
Cucumber mosaic virus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.7.7.48 |
-15°C, buffer containing 30% glycerol, complete loss of activity after 2-3 days |
Cucumber mosaic virus |
2.7.7.48 |
0°C, buffer containing 30% glycerol, complete loss of activity after 1-2 days |
Cucumber mosaic virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
GTP + CMV RNA, yeast RNA or poly(C) |
Cucumber mosaic virus |
diphosphate + RNAn+1 |
- |
? |
|