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Literature summary extracted from

  • Warner, A.H.; Beers, P.C.; Huang, F.L.
    Biosynthesis of the diguanosine nucleotides. I. Purification and properties of an enzyme from yolk platelets of brine shrimp embryos (1974), Can. J. Biochem., 52, 231-240.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.45 DTT required Artemia salina

General Stability

EC Number General Stability Organism
2.7.7.45 albumin, 10 mg/ml, glycerol, 30% or P1,P4-bis(5'-guanosyl)tetraphosphate, 0.6 mM, prevents loss of activity at 0°C, Gp4G most effective Artemia salina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.45 diphosphate
-
 Artemia salina
2.7.7.45 additional information no inhibition by phosphate Artemia salina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.45 Cu2+ 28% of the activity with Mg2+ in P1,P4-bis(5'-guanosyl)tetraphosphate synthesis Artemia salina
2.7.7.45 Mg2+ 20 mM required with GDP as substrate Artemia salina
2.7.7.45 Mg2+ 10-15 mM required for maximal activity with GTP as substrate Artemia salina
2.7.7.45 Mn2+ 86% of the activity with Mg2+ in P1,P4-bis(5'-guanosyl)tetraphosphate synthesis Artemia salina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.45 490000
-
 gel filtration Artemia salina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.45 GTP + GTP Artemia salina enzyme catalyzes synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate during oogenesis in Artemia diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.45 Artemia salina
-
 brine shrimp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.45 partial Artemia salina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.45 embryo encysted, yolk platelets of Artemia salina
-
2.7.7.45 platelet of yolk Artemia salina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.45 additional information
-
-
 Artemia salina
2.7.7.45 0.003 0.0066 partially purified enzyme Artemia salina

Storage Stability

EC Number Storage Stability Organism
2.7.7.45 0°C or -15°C, 50% loss of activity after 1 week in buffered 500 mM NaCl Artemia salina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.45 GDP + GDP rate of the reaction is low compared with synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate and dependent on other small molecular weight components of yolk platelets Artemia salina P1,P3-bis(5'-guanosyl)triphosphate + diphosphate i.e. GP3G r
2.7.7.45 GTP + GTP
-
 Artemia salina P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate i.e. GP4G r
2.7.7.45 GTP + GTP enzyme catalyzes synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate during oogenesis in Artemia Artemia salina diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.45 37
-
 P1,P3-bis(5'-guanosyl)triphosphate synthesis, partially purified enzyme, Sephadex G-25 fraction Artemia salina
2.7.7.45 40 42 P1,P4-bis(5'-guanosyl)tetraphosphate synthesis Artemia salina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.45 5.9 6
-
 Artemia salina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.45 4.5 8 no activity below and above Artemia salina