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Literature summary extracted from

  • Rhee, S.G.; Park, R.; Wittenberger, M.
    New enzymic assays for glutamine synthetase adenylytransferase and its regulatory protein PIIA (1978), Anal. Biochem., 88, 174-185.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.42 L-glutamine required Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 L-glutamine required Escherichia coli
2.7.7.42 L-glutamine required Pseudomonas putida
2.7.7.42 PII regulatory protein PIIA is required Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 PII regulatory protein PIIA is required Escherichia coli
2.7.7.42 PII regulatory protein PIIA is required Pseudomonas putida
2.7.7.42 PII regulatory protein activity is modulated by a regulatory protein PII, which exists in two interconvertible forms, PIIA and PIID, the unmodified form PIIA stimulates the adenylylation of [L-glutamate:ammonia ligase (ADP-forming)] , the uridylated form PIID is required for deadenylylation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 PII regulatory protein activity is modulated by a regulatory protein PII, which exists in two interconvertible forms, PIIA and PIID, the unmodified form PIIA stimulates the adenylylation of [L-glutamate:ammonia ligase (ADP-forming)] , the uridylated form PIID is required for deadenylylation Escherichia coli
2.7.7.42 PII regulatory protein activity is modulated by a regulatory protein PII, which exists in two interconvertible forms, PIIA and PIID, the unmodified form PIIA stimulates the adenylylation of [L-glutamate:ammonia ligase (ADP-forming)] , the uridylated form PIID is required for deadenylylation Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.42 Mg2+ activates Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 Mg2+ activates Escherichia coli
2.7.7.42 Mg2+ activates Pseudomonas putida
2.7.7.42 Mn2+ activates, in presence of PIIA Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 Mn2+ activates, in presence of PIIA Escherichia coli
2.7.7.42 Mn2+ activates, in presence of PIIA Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)] Salmonella enterica subsp. enterica serovar Typhimurium
-
 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)] Escherichia coli
-
 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)] Pseudomonas putida
-
 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.42 Escherichia coli
-
 W
-
2.7.7.42 Pseudomonas putida
-
-
-
2.7.7.42 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.42 partial Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 partial Escherichia coli
2.7.7.42 partial Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.42 additional information
-
 assay method Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 additional information
-
 assay method Escherichia coli
2.7.7.42 additional information
-
 assay method Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Pseudomonas putida diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
2.7.7.42 ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Pseudomonas putida diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.42 ATASE
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 ATASE
-
 Escherichia coli
2.7.7.42 ATASE
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.42 26
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 26
-
 assay at Escherichia coli
2.7.7.42 26
-
 assay at Pseudomonas putida
2.7.7.42 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.42 7.5
-
 about, assay at Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 7.5
-
 about, assay at Escherichia coli
2.7.7.42 7.5
-
 about, assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.42 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.42 ATP
-
 Escherichia coli
2.7.7.42 ATP
-
 Pseudomonas putida