BRENDA - Enzyme Database

Biosynthesis of CDP-diacylglycerol in hog mesenteric lymph node lymphocytes

Sribney, M.; Hegadorn, C.A.; J. Biochem. 60, 668-674 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
ATP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
cationic detergent
the phosphatidate must be emulsified in a cationic detergent for optimal activity
Sus scrofa
2.7.7.41
GTP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
ITP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
lecithin
stimulates
Sus scrofa
2.7.7.41
sphingomyelin
stimulates
Sus scrofa
2.7.7.41
UTP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
General Stability
EC Number
General Stability
Organism
2.7.7.41
-20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
Ca2+
2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
cardiolipin
2 mM, 27% inhibition
Sus scrofa
2.7.7.41
Co2+
2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
F-
20 mM NaF, 70% inhibition
Sus scrofa
2.7.7.41
FeSO4
2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
lysolecithin
1 mM, 89% inhibition
Sus scrofa
2.7.7.41
oleic acid
2 mM, 84% inhibition
Sus scrofa
2.7.7.41
palmitic acid
2 mM, 76% inhibition
Sus scrofa
2.7.7.41
palmitoyl-CoA
0.5 mM, 70% inhibition
Sus scrofa
2.7.7.41
phosphatidylethanolamine
2 mM, 16% inhibition
Sus scrofa
2.7.7.41
phosphatidylinositol
2 mM, 23% inhibition
Sus scrofa
2.7.7.41
phosphatidylserine
2 mM, 46% inhibition
Sus scrofa
2.7.7.41
SDS
-
Sus scrofa
2.7.7.41
sodium deoxycholate
-
Sus scrofa
2.7.7.41
Triton X-100
-
Sus scrofa
2.7.7.41
Tween 20
-
Sus scrofa
2.7.7.41
ZWT-12
2 mM, 86% inhibition
Sus scrofa
2.7.7.41
ZWT-14
2 mM, 92% inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.6
-
CTP
pH 7.0, 37°C
Sus scrofa
2.7.7.41
0.65
-
phosphatidate
pH 7.0, 37°C
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
maximal stimulation at 20 mM
Sus scrofa
2.7.7.41
Mn2+
up to 10 mM, never more than 15% as effective as Mg2+
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Sus scrofa
-
hog
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
lymphocyte
-
Sus scrofa
-
2.7.7.41
mesenteric lymph node
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.00011
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.41
-20°C, 24 h, enzyme in isolated microsomes, 75% loss of activity
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
activity with phosphatidate obtained from egg lecithin which has a variety of mixed fatty acid in ester linkage. The phosphatidate has two oleoyl fatty acids is only 20% as effective as the phospatidate prepared from egg lecithin
643311
Sus scrofa
diphosphate + CDPdiacylglycerol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7
-
Tris-HCl buffer
Sus scrofa
2.7.7.41
7.5
-
phosphate buffer
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
ATP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
cationic detergent
the phosphatidate must be emulsified in a cationic detergent for optimal activity
Sus scrofa
2.7.7.41
GTP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
ITP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
2.7.7.41
lecithin
stimulates
Sus scrofa
2.7.7.41
sphingomyelin
stimulates
Sus scrofa
2.7.7.41
UTP
stimulates only in presence of either lecithin or sphingomyelin
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.41
-20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
Ca2+
2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
cardiolipin
2 mM, 27% inhibition
Sus scrofa
2.7.7.41
Co2+
2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
F-
20 mM NaF, 70% inhibition
Sus scrofa
2.7.7.41
FeSO4
2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations
Sus scrofa
2.7.7.41
lysolecithin
1 mM, 89% inhibition
Sus scrofa
2.7.7.41
oleic acid
2 mM, 84% inhibition
Sus scrofa
2.7.7.41
palmitic acid
2 mM, 76% inhibition
Sus scrofa
2.7.7.41
palmitoyl-CoA
0.5 mM, 70% inhibition
Sus scrofa
2.7.7.41
phosphatidylethanolamine
2 mM, 16% inhibition
Sus scrofa
2.7.7.41
phosphatidylinositol
2 mM, 23% inhibition
Sus scrofa
2.7.7.41
phosphatidylserine
2 mM, 46% inhibition
Sus scrofa
2.7.7.41
SDS
-
Sus scrofa
2.7.7.41
sodium deoxycholate
-
Sus scrofa
2.7.7.41
Triton X-100
-
Sus scrofa
2.7.7.41
Tween 20
-
Sus scrofa
2.7.7.41
ZWT-12
2 mM, 86% inhibition
Sus scrofa
2.7.7.41
ZWT-14
2 mM, 92% inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
0.6
-
CTP
pH 7.0, 37°C
Sus scrofa
2.7.7.41
0.65
-
phosphatidate
pH 7.0, 37°C
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
maximal stimulation at 20 mM
Sus scrofa
2.7.7.41
Mn2+
up to 10 mM, never more than 15% as effective as Mg2+
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.7.41
lymphocyte
-
Sus scrofa
-
2.7.7.41
mesenteric lymph node
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
0.00011
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.41
-20°C, 24 h, enzyme in isolated microsomes, 75% loss of activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + phosphatidate
activity with phosphatidate obtained from egg lecithin which has a variety of mixed fatty acid in ester linkage. The phosphatidate has two oleoyl fatty acids is only 20% as effective as the phospatidate prepared from egg lecithin
643311
Sus scrofa
diphosphate + CDPdiacylglycerol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
7
-
Tris-HCl buffer
Sus scrofa
2.7.7.41
7.5
-
phosphate buffer
Sus scrofa