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Literature summary extracted from

  • Kelley, M.J.; Carman, G.M.
    Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae (1987), J. Biol. Chem., 262, 14563-14570.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.41 Triton X-100 maximal stimulation at 15 mM Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.7.7.41 stable to at least 2 cycles of freezing and thawing Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 dCTP competitive with respect to CTP Saccharomyces cerevisiae
2.7.7.41 Hg2+ 5 mM HgCl2, 78% inhibition Saccharomyces cerevisiae
2.7.7.41 PCMB 5 mM, 88% inhibition Saccharomyces cerevisiae
2.7.7.41 Triton X-100 above 20 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.5
-
 phosphatidate pH 6.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 mitochondrial membrane
-
 Saccharomyces cerevisiae 31966
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.41 Mg2+ maximal activity at 20 mM Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.41 114000
-
 radiation inactivation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.41 CTP + phosphatidate = diphosphate + CDP-diacylglycerol sequential bi bi reaction mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.41 1.409
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.7.41 -80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate equilibrium constant is 0.001. Reverse reaction is favored in vitro Saccharomyces cerevisiae diphosphate + CDPdiacylglycerol
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.41 More two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.41 30
-
 20 min, labile above Saccharomyces cerevisiae
2.7.7.41 60
-
 20 min, complete inactivation Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.41 8.92
-
 CTP pH 6.5, 30°C Saccharomyces cerevisiae
2.7.7.41 8.92
-
 phosphatidate pH 6.5, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 6.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.41 6 8.5 pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity Saccharomyces cerevisiae