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Literature summary extracted from

  • Gaillard, J.L.; Lubochinsky, B.; Rigomier, D.
    Specific inhibition of phosphatidate cytidylyltransferase from Bacillus subtilis membranes by cytidine monophosphate (1983), Biochim. Biophys. Acta, 753, 372-380.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.41 non-ionic detergent absolute requirement Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 CMP
-
 Bacillus subtilis
2.7.7.41 diphosphate
-
 Bacillus subtilis
2.7.7.41 Hg2+ 0.1 mM, 90% inhibition Bacillus subtilis
2.7.7.41 Mg2+ 33% inhibition at 25 mM Mg2+ Bacillus subtilis
2.7.7.41 PCMB 0.03 mM, 90% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.26
-
 CTP pH 7.5, 25°C Bacillus subtilis
2.7.7.41 0.26
-
 dCTP pH 7.5, 25°C Bacillus subtilis
2.7.7.41 0.3
-
 phosphatidic acid pH 7.5, 25°C, with 0.45 mM CTP Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 membrane localized exclusively in the membrane fraction Bacillus subtilis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.41 K+ stimulating effect increases with concentrations up to 75 mM Bacillus subtilis
2.7.7.41 Mg2+ maximal activity with 3-6 mM. 33% inhibition at 25 mM Bacillus subtilis
2.7.7.41 Mn2+ 1 mM, 70% of the maximal activity obtained with Mg2+ Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Bacillus subtilis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.41 CTP + phosphatidate = diphosphate + CDP-diacylglycerol ping-pong mechanism Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + 1,2-dipalmitoyl phosphatidic acid
-
 Bacillus subtilis diphosphate + CDP-dipalmitoylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate
-
 Bacillus subtilis diphosphate + CDPdiacylglycerol
-
 ?
2.7.7.41 dCTP + phosphatidate
-
 Bacillus subtilis diphosphate + dCDPdiacylglycerol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.41 40
-
 with detergent already inactivated at Bacillus subtilis
2.7.7.41 60
-
 30 min, membranous enzyme, stable up to Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 7.5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.41 6.2 8.7 pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.41 0.75
-
 diphosphate pH 7.5, 25°C Bacillus subtilis
2.7.7.41 5.6
-
 CMP pH 7.5, 25°C Bacillus subtilis