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Literature summary extracted from

  • Sperling, D.; Kappler, U.; Wynen, A.; Dahl, C.; Truper, H.G.
    Dissimilatory ATP sulfurylase from the hyperthermophilic sulfate reducer Archaeoglobus fulgidus belongs to the group of homo-oligomeric ATP sulfurylases (1998), FEMS Microbiol. Lett., 162, 257-264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 overexpression in Eacherichia coli Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.13
-
 diphosphate
-
 Archaeoglobus fulgidus
2.7.7.4 0.17
-
 adenosine 5'-phosphosulfate
-
 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 57000
-
 2 * 57000, SDS-PAGE Archaeoglobus fulgidus
2.7.7.4 150000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 ATP + sulfate Archaeoglobus fulgidus constitutive enzyme diphosphate + adenylylsulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Archaeoglobus fulgidus
-
 DSM 4304T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 recombinant enzyme Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 1.1
-
 cell extract Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Archaeoglobus fulgidus diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate constitutive enzyme Archaeoglobus fulgidus diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 dimer 2 * 57000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.4 90
-
 wild-type enzyme Archaeoglobus fulgidus
2.7.7.4 100
-
 recombinant enzyme Archaeoglobus fulgidus