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Literature summary extracted from

  • Hanna, E.; MacRae, I.J.; Medina, D.C.; Fisher, A.J.; Segel, I.H.
    ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus (2002), Arch. Biochem. Biophys., 406, 275-288.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 expression in Escherichia coli BL-21 Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 ClO3-
-
 Aquifex aeolicus
2.7.7.4 ClO4- competitive with sulfate and adenylyl sulfate Aquifex aeolicus
2.7.7.4 FSO3-
-
 Aquifex aeolicus
2.7.7.4 NO3-
-
 Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.0051
-
 adenosine 5'-phosphosulfate pH 8.0, 37°C Aquifex aeolicus
2.7.7.4 0.057
-
 diphosphate pH 8.0, 30°C Aquifex aeolicus
2.7.7.4 0.15
-
 MoO42- pH 8.0, 30°C Aquifex aeolicus
2.7.7.4 0.23
-
 MgATP2- pH 8.0, 30°C, reaction with MoO42- Aquifex aeolicus
2.7.7.4 1.1
-
 MgATP2- pH 8.0, 30°C, reaction with SO42- Aquifex aeolicus
2.7.7.4 3.3
-
 SO42- pH 8.0, 30°C Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 62800
-
 4 * 62800, calculation from amino acid sequence Aquifex aeolicus
2.7.7.4 260000
-
 gel filtration Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 additional information Aquifex aeolicus the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Aquifex aeolicus O67174
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + MoO42-
-
 Aquifex aeolicus AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + sulfate
-
 Aquifex aeolicus diphosphate + adenylylsulfate
-
 r
2.7.7.4 additional information the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation Aquifex aeolicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.4 tetramer 4 * 62800, calculation from amino acid sequence Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.4 90
-
 half-life: more than 1 h Aquifex aeolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 7 8.5
-
 Aquifex aeolicus