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Literature summary extracted from

  • Osslund, T.; Chandler, C.; Segel, I.H.
    ATP sulfurylase from higher plants. Purification and preliminary kinetics studies on the cabbage leaf enzyme (1982), Plant Physiol., 70, 39-45.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 adenosine 5'-phosphosulfate
-
Brassica capitata
2.7.7.4 ClO3-
-
Brassica capitata
2.7.7.4 MgATP2-
-
Brassica capitata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.00071
-
diphosphate pH 8.0, 30°C Brassica capitata
2.7.7.4 0.001
-
adenosine 5'-phosphosulfate pH 8.0, 30°C Brassica capitata
2.7.7.4 0.31
-
ATP pH 8.0, 30°C, SO42- as substrate Brassica capitata
2.7.7.4 0.31
-
ATP ATP in form of MgATP2- Brassica capitata
2.7.7.4 0.33
-
ATP pH 8.0, 30°C, MoO42- as substrate Brassica capitata
2.7.7.4 0.33
-
ATP ATP in form of MgATP2- Brassica capitata
2.7.7.4 0.64
-
MoO42- pH 8.0, 30°C Brassica capitata
2.7.7.4 0.87
-
SO42- pH 8.0, 30°C Brassica capitata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 108000
-
glycerol density gradient centrifugation Brassica capitata

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Brassica capitata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
Brassica capitata

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.4 ATP + sulfate = diphosphate + adenylyl sulfate obligatory ordered kinetic mechanism with MgATP2- adding before MoO42- or SO42- and Mg-diphosphate leaving before AMP + MoO42- or adenosine 5'-phosphosulfate Brassica capitata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 leaf
-
Brassica capitata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 3.3
-
synthesis of adenosine 5'-phosphosulfate Brassica capitata
2.7.7.4 4.3
-
Mg-diphosphate-MgATP2-exchange Brassica capitata
2.7.7.4 30
-
-
Brassica capitata
2.7.7.4 38
-
molybdolysis, and synthesis of MgATP2- Brassica capitata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + molybdate
-
Brassica capitata diphosphate + adenylyl molybdate
-
r
2.7.7.4 ATP + MoO42-
-
Brassica capitata AMP + adenylylmolybdate
-
?
2.7.7.4 ATP + sulfate
-
Brassica capitata diphosphate + adenylylsulfate
-
r
2.7.7.4 ATP + sulfate
-
Brassica capitata diphosphate + adenylyl sulfate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 dimer 2 * 56000-57000, 2 band of 56000 Da and of 57000 Da, the 56000 Da protein perhaps is a partially proteolyzed subunit, SDS-PAGE Brassica capitata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.4 3.13
-
ATP pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate Brassica capitata
2.7.7.4 3.13
-
SO42- pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate Brassica capitata
2.7.7.4 36
-
ATP pH 8.0, 30°C, molybdolysis Brassica capitata
2.7.7.4 36
-
diphosphate pH 8.0, 30°C, synthesis of ATP Brassica capitata
2.7.7.4 36
-
adenosine 5'-phosphosulfate pH 8.0, 30°C, synthesis of ATP Brassica capitata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8
-
-
Brassica capitata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.4 0.0015
-
adenosine 5'-phosphosulfate pH 8.0, 30°C, product inhibitor Brassica capitata
2.7.7.4 0.3
-
ClO3- pH 8.0, 30°C Brassica capitata
2.7.7.4 0.75 1.3 MgATP2- pH 8.0, 30°C Brassica capitata