EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.4 | adenosine 5'-phosphosulfate | - |
Brassica capitata | |
2.7.7.4 | ClO3- | - |
Brassica capitata | |
2.7.7.4 | MgATP2- | - |
Brassica capitata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 0.00071 | - |
diphosphate | pH 8.0, 30°C | Brassica capitata | |
2.7.7.4 | 0.001 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Brassica capitata | |
2.7.7.4 | 0.31 | - |
ATP | pH 8.0, 30°C, SO42- as substrate | Brassica capitata | |
2.7.7.4 | 0.31 | - |
ATP | ATP in form of MgATP2- | Brassica capitata | |
2.7.7.4 | 0.33 | - |
ATP | pH 8.0, 30°C, MoO42- as substrate | Brassica capitata | |
2.7.7.4 | 0.33 | - |
ATP | ATP in form of MgATP2- | Brassica capitata | |
2.7.7.4 | 0.64 | - |
MoO42- | pH 8.0, 30°C | Brassica capitata | |
2.7.7.4 | 0.87 | - |
SO42- | pH 8.0, 30°C | Brassica capitata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 108000 | - |
glycerol density gradient centrifugation | Brassica capitata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.4 | Brassica capitata | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.4 | - |
Brassica capitata |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.4 | ATP + sulfate = diphosphate + adenylyl sulfate | obligatory ordered kinetic mechanism with MgATP2- adding before MoO42- or SO42- and Mg-diphosphate leaving before AMP + MoO42- or adenosine 5'-phosphosulfate | Brassica capitata |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.4 | leaf | - |
Brassica capitata | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 3.3 | - |
synthesis of adenosine 5'-phosphosulfate | Brassica capitata |
2.7.7.4 | 4.3 | - |
Mg-diphosphate-MgATP2-exchange | Brassica capitata |
2.7.7.4 | 30 | - |
- |
Brassica capitata |
2.7.7.4 | 38 | - |
molybdolysis, and synthesis of MgATP2- | Brassica capitata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.4 | ATP + molybdate | - |
Brassica capitata | diphosphate + adenylyl molybdate | - |
r | |
2.7.7.4 | ATP + MoO42- | - |
Brassica capitata | AMP + adenylylmolybdate | - |
? | |
2.7.7.4 | ATP + sulfate | - |
Brassica capitata | diphosphate + adenylylsulfate | - |
r | |
2.7.7.4 | ATP + sulfate | - |
Brassica capitata | diphosphate + adenylyl sulfate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.4 | dimer | 2 * 56000-57000, 2 band of 56000 Da and of 57000 Da, the 56000 Da protein perhaps is a partially proteolyzed subunit, SDS-PAGE | Brassica capitata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 3.13 | - |
ATP | pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate | Brassica capitata | |
2.7.7.4 | 3.13 | - |
SO42- | pH 8.0, 30°C, synthesis of adenosine 5'-phosphosulfate | Brassica capitata | |
2.7.7.4 | 36 | - |
ATP | pH 8.0, 30°C, molybdolysis | Brassica capitata | |
2.7.7.4 | 36 | - |
diphosphate | pH 8.0, 30°C, synthesis of ATP | Brassica capitata | |
2.7.7.4 | 36 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C, synthesis of ATP | Brassica capitata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 8 | - |
- |
Brassica capitata |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 0.0015 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C, product inhibitor | Brassica capitata | |
2.7.7.4 | 0.3 | - |
ClO3- | pH 8.0, 30°C | Brassica capitata | |
2.7.7.4 | 0.75 | 1.3 | MgATP2- | pH 8.0, 30°C | Brassica capitata |