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Literature summary extracted from

  • Dahl, C.; Koch, H.G.; Keuken, O.; Trüper, H.G.
    Purification and characterization of ATP sulfurylase from the extremely thermophilic archaebacterial sulfate-reducer, Archaeoglobus fulgidus (1990), FEMS Microbiol. Lett., 67, 27-32.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.13
-
 diphosphate pH 7.5, 85°C Archaeoglobus fulgidus
2.7.7.4 0.17
-
 adenosine 5'-phosphosulfate pH 7.5, 85°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 50000
-
 alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE Archaeoglobus fulgidus
2.7.7.4 53000
-
 alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE Archaeoglobus fulgidus
2.7.7.4 150000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 33.9
-
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Archaeoglobus fulgidus diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 trimer alpha2beta, 2 * 50000 + 1 * 53000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.4 90
-
-
 Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8
-
-
 Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 5.5 9 in Tris-malate and Tris-HCl buffer active between pH 5.5 and pH 9.0 Archaeoglobus fulgidus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.4 Archaeoglobus fulgidus isoelectric focusing 4.4 4.3