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Literature summary extracted from

  • Stern, A.I.; Avron, M.
    An adenosine 5-diphosphate ribose:orthophosphate adenylyltransferase from Euglena gracilis (1966), Biochim. Biophys. Acta, 118, 577-591.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.35 additional information no activation by EDTA Euglena gracilis

General Stability

EC Number General Stability Organism
2.7.7.35 solution of lyophilized enzyme, 2 mg/ml, stable to repeated freeze-thaw cycles during 2 weeks Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.35 ADP weak, ADP-ribose phosphorolysis Euglena gracilis
2.7.7.35 AMP kinetics Euglena gracilis
2.7.7.35 arsenate kinetics Euglena gracilis
2.7.7.35 ATP kinetics Euglena gracilis
2.7.7.35 D-ribose 5-phosphate kinetics Euglena gracilis
2.7.7.35 IDP weak, ADPribose phosphorolysis Euglena gracilis
2.7.7.35 additional information no inhibition by adenosine, deoxyadenosine, 3'-AMP, CMP, CDP, UDP, NADH, NAD+, ADPglucose, UDPglucose, IAA, NEM; no inhibition by NADP+, EDTA, ribose Euglena gracilis
2.7.7.35 PCMB ADP/phosphate-exchange and ADPribose phosphorolysis Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.35 0.05
-
 ADP-ribose pH 7.8, 35°C Euglena gracilis
2.7.7.35 0.5
-
 phosphate pH 7.8, 35°C Euglena gracilis
2.7.7.35 0.6
-
 ADP ADP/phosphate exchange Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.35 cytosol
-
 Euglena gracilis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.35 additional information no Mn2+ or Mg2+ requirement Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.35 phosphate + ADP-ribose Euglena gracilis
-
 ADP + D-ribose 5-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.35 Euglena gracilis
-
 var. bacillaris Pringsheim
-
2.7.7.35 no activity in Chlorella pyrenoidosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.35 partial Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.35 cell culture heterotrophically or autotrophically grown cells Euglena gracilis
-
2.7.7.35 cell culture UV-mutant cells incapable of forming chloroplasts Euglena gracilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.35 0.51
-
 partially purified enzyme Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.7.7.35 -15°C, undialyzed purified enzyme, lyophilized and placed under vacuum, 35% loss of activity within 2 months Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.35 additional information no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose Euglena gracilis ?
-
 ?
2.7.7.35 additional information no activity with AMP and ATP Euglena gracilis ?
-
 ?
2.7.7.35 phosphate + ADP ADP/phosphate-exchange reaction, inorganic phosphate is incorporated into terminal position of ADP Euglena gracilis ADP + phosphate
-
 r
2.7.7.35 phosphate + ADP IDP can replace ADP with 16% efficiency Euglena gracilis ADP + phosphate
-
 r
2.7.7.35 phosphate + ADP-ribose
-
 Euglena gracilis ADP + D-ribose 5-phosphate
-
 ir
2.7.7.35 phosphate + ADP-ribose highly specific Euglena gracilis ADP + D-ribose 5-phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.35 50
-
-
 Euglena gracilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.35 29 56 about half-maximal activity at 29°C and 56°C Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.35 60
-
 3 min, inactivation Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.35 7.5
-
 ADP/phosphate-exchange Euglena gracilis
2.7.7.35 8
-
 ADP-ribose phosphorolysis Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.35 6.7 8.5 ADP/phosphate-exchange, about 60% of maximal activity at pH 6.7 and about half-maximal activity at pH 8.5 Euglena gracilis
2.7.7.35 6.8 9.4 about half-maximal activity at pH 6.8 and pH 9.4 Euglena gracilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.35 0.09
-
 D-ribose 5-phosphate phosphorolysis Euglena gracilis
2.7.7.35 0.09
-
 D-ribose 5-phosphate pH 7.8, 35°C Euglena gracilis
2.7.7.35 0.11
-
 D-ribose 5-phosphate pH 7.8, 35°C Euglena gracilis
2.7.7.35 0.11
-
 D-ribose 5-phosphate ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 0.41
-
 arsenate pH 7.8, 35°C Euglena gracilis
2.7.7.35 0.41
-
 arsenate ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 0.57
-
 arsenate phosphorolysis Euglena gracilis
2.7.7.35 0.57
-
 arsenate pH 7.8, 35°C Euglena gracilis
2.7.7.35 1
-
 AMP phosphorolysis Euglena gracilis
2.7.7.35 1
-
 AMP pH 7.8, 35°C Euglena gracilis
2.7.7.35 1.3
-
 ATP pH 7.8, 35°C Euglena gracilis
2.7.7.35 1.3
-
 ATP ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 1.5
-
 AMP pH 7.8, 35°C Euglena gracilis
2.7.7.35 1.5
-
 AMP ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 2.9
-
 ATP phosphorolysis Euglena gracilis
2.7.7.35 2.9
-
 ATP pH 7.8, 35°C Euglena gracilis