EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.33 | additional information | no activation by dTDP-L-rhamnose, dTDP-4-amino-4,6-dideoxy-D-glucose and its N-acetyl-derivative, UDP-D-galactose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, GDP-D-mannose, D-glucose 6-phosphate, D-glucose 1,6-diphosphate, D-galactose 1-phosphate, D-glucosamine 1-phosphate, D-mannose 1-phosphate, N-acetyl-D-glucosamine 1-phosphate, D-fructose 6-phosphate, D-fructose 1,6-diphosphate, D-ribitol 5-phosphate, L-glycerol 3-phosphate, phosphoenolpyruvate, pyruvate, ADP, AMP, GMP, UMP, beta-NAD+ or beta-NADP+ | Azotobacter vinelandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.33 | ADPglucose | weak | Azotobacter vinelandii | |
2.7.7.33 | ATP | - |
Azotobacter vinelandii | |
2.7.7.33 | CDP | weak | Azotobacter vinelandii | |
2.7.7.33 | CDP-2-O-methyldeoxyaldose | - |
Azotobacter vinelandii | |
2.7.7.33 | CDP-D-galactose | weak | Azotobacter vinelandii | |
2.7.7.33 | CDPglucose | product inhibition, competitive with respect to CTP, kinetics | Azotobacter vinelandii | |
2.7.7.33 | dADP | weak | Azotobacter vinelandii | |
2.7.7.33 | dATP | - |
Azotobacter vinelandii | |
2.7.7.33 | dGTP | - |
Azotobacter vinelandii | |
2.7.7.33 | diphosphate | product inhibition, kinetics | Azotobacter vinelandii | |
2.7.7.33 | dTDP | weak | Azotobacter vinelandii | |
2.7.7.33 | dTTP | strong, competitive with CTP, kinetics | Azotobacter vinelandii | |
2.7.7.33 | GTP | - |
Azotobacter vinelandii | |
2.7.7.33 | additional information | little or no inhibition by CDPethanolamine, CDPcholine, dTTPglucose, UDP-D-glucosamine, dCTP, dCDP, dTMP, dAMP, dGMP, dCMP, dTDP-L-rhamnose, dTDP-4-amino-4,6-dideoxy-D-glucose and its N-acetyl-derivative, UDP-D-galactose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, GDP-D-mannose, D-glucose 6-phosphate, D-glucose 1,6-diphosphate, D-galactose 1-phosphate, D-glucosamine 1-phosphate, D-mannose 1-phosphate, N-acetyl-D-glucosamine 1-phosphate, D-fructose 6-phosphate, D-fructose 1,6-diphosphate, D-ribitol 5-phosphate, L-glycerol 3-phosphate, phosphoenolpyruvate, pyruvate, ADP, AMP, GMP, UMP, beta-NAD+ or beta-NADP+; little or no inhibition by CMP, GDPglucose, UDPglucose, dTDPglucose | Azotobacter vinelandii | |
2.7.7.33 | phosphate | weak | Azotobacter vinelandii | |
2.7.7.33 | UTP | - |
Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.33 | 0.56 | - |
diphosphate | pH 8.5, 37°C | Azotobacter vinelandii | |
2.7.7.33 | 0.56 | - |
diphosphate | diphosphate in form of magnesium diphosphate | Azotobacter vinelandii | |
2.7.7.33 | 5 | - |
D-glucosamine 1-phosphate | pH 8.5, 37°C | Azotobacter vinelandii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.33 | Mg2+ | requirement | Azotobacter vinelandii | |
2.7.7.33 | Mg2+ | maximal reaction rates at molar ratios of MgCl2:diphosphate or MgCl2:CTP of 2:1 | Azotobacter vinelandii | |
2.7.7.33 | Mn2+ | requirement | Azotobacter vinelandii | |
2.7.7.33 | Mn2+ | can replace Mg2+ with 87% efficiency | Azotobacter vinelandii | |
2.7.7.33 | additional information | no activation by Ni2+ | Azotobacter vinelandii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.33 | Azotobacter vinelandii | - |
- |
- |
2.7.7.33 | Azotobacter vinelandii O | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.33 | partial | Azotobacter vinelandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.33 | 0.0375 | - |
- |
Azotobacter vinelandii |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.33 | -18°C, in EDTA-containing Tris-HCl buffer, at least 1 year | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | highly specific | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: dTTP, alpha-D-galactose 1-phosphate | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: ATP, GTP | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: dATP, dGTP, dCTP, D-ribitol 5-phosphate, L-glycerol 3-phosphate | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrates in the reverse reaction: GDPglucose | Azotobacter vinelandii | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | highly specific | Azotobacter vinelandii O | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrates in the reverse reaction: ADPglucose, dTDPglucose, UDPglucose | Azotobacter vinelandii O | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: alpha-D-mannose 1-phosphate, N-acetyl-alpha-D-glucosamine 1-phosphate | Azotobacter vinelandii O | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: dTTP, alpha-D-galactose 1-phosphate | Azotobacter vinelandii O | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + alpha-D-glucose 1-phosphate | no substrate in the forward reaction: ATP, GTP | Azotobacter vinelandii O | diphosphate + CDPglucose | - |
r | |
2.7.7.33 | CTP + D-glucosamine 1-phosphate | reaction at about 25% the rate with glucose 1-phosphate | Azotobacter vinelandii | diphosphate + CDPglucosamine | - |
? | |
2.7.7.33 | diphosphate + CDP-glucose | - |
Azotobacter vinelandii | CTP + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.33 | 37 | - |
assay at | Azotobacter vinelandii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.33 | 8.3 | - |
diphosphorolysis | Azotobacter vinelandii |
2.7.7.33 | 8.5 | - |
CDPglucose synthesis | Azotobacter vinelandii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.33 | 0.04 | - |
dTTP | pH 8.5, 37°C | Azotobacter vinelandii | |
2.7.7.33 | 0.3 | - |
diphosphate | pH 8.5, 37°C | Azotobacter vinelandii | |
2.7.7.33 | 0.35 | - |
CDPglucose | pH 8.5, 37°C | Azotobacter vinelandii |