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Literature summary extracted from

  • Ono, K.
    Inhibitory effects of various 2,3-dideoxynucleoside 5-triphosphates on the utilization of 2-deoxynucleoside 5-triphosphates by terminal deoxynucleotidyltransferase from calf thymus (1990), Biochim. Biophys. Acta, 1049, 15-20.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.31 2',3'-dideoxyadenosine inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate ATP, 50% inhibition at 0.006 mM Bos taurus
2.7.7.31 2',3'-dideoxycytidine inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate CTP, 50% inhibition at 0.002 mM Bos taurus
2.7.7.31 2',3'-dideoxyguanosine inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate GTP, 50% inhibition at 0.005 mM Bos taurus
2.7.7.31 2',3'-dideoxythymidine inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate TTP, 50% inhibition at 0.020 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.31 0.004
-
 dATP 37°C, primer (dA)12-18 Bos taurus
2.7.7.31 0.071
-
 dCTP 37°C, primer (dA)12-18 Bos taurus
2.7.7.31 0.114
-
 dGTP 37°C, primer (dA)12-18 Bos taurus
2.7.7.31 0.248
-
 dTTP 37°C, primer (dA)12-18 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.31 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 dATP + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 dCTP + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn primer required Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 deoxynucleoside triphosphate + DNAn reaction is not template directed Bos taurus diphosphate + DNAn+1
-
 ?
2.7.7.31 dGTP + DNAn
-
 Bos taurus diphosphate + DNAn+1
-
 ?