Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aghajanian, S.; Worrall, D.M.
    Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase) (2002), Biochem. J., 365, 13-18.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.24 gene ppat/dpck encoding bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activity, located on chromosome 17q12-21, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) Homo sapiens
2.7.7.3 expression in Escherichia coli BL21 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.24 0.0041
-
 dephospho-CoA native enzyme, pH 8.0, 25°C Sus scrofa
2.7.1.24 0.0052
-
 dephospho-CoA recombinant enzyme, pH 8.0, 25°C Homo sapiens
2.7.1.24 0.19
-
 ATP recombinant enzyme, pH 8.0, 25°C Homo sapiens
2.7.1.24 0.33
-
 ATP recombinant enzyme, pH 8.0, 25°C Sus scrofa
2.7.7.3 0.0147
-
 3'-dephospho-CoA 25°C, pH 8 Sus scrofa
2.7.7.3 0.272
-
 diphosphate 25°C, pH 8 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.24 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.24 60000
-
 1 * 60000, SDS-PAGE Sus scrofa
2.7.1.24 62000
-
 recombinant enzyme, gel filtration Homo sapiens
2.7.1.24 62000
-
 wild-type enzyme, gel filtration Sus scrofa
2.7.7.3 62000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.24 Homo sapiens Q13057 gene ppat/dpck, bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities, termed CoA synthase
-
2.7.1.24 Sus scrofa Q8MIR4 bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities
-
2.7.7.3 Homo sapiens Q13057
-
-
2.7.7.3 Sus scrofa Q8MIR4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.24
-
 Sus scrofa
2.7.1.24 recombinant from Escherichia coli Homo sapiens
2.7.7.3 to homogenity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.24 Hep-G2 cell
-
 Homo sapiens
-
2.7.1.24 liver
-
 Sus scrofa
-
2.7.7.3 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.24 ATP + dephospho-CoA
-
 Homo sapiens ADP + CoA
-
 ?
2.7.1.24 ATP + dephospho-CoA
-
 Sus scrofa ADP + CoA
-
 ?
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Homo sapiens diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Sus scrofa diphosphate + 3'-dephospho-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.24 monomer 1 * 60000, SDS-PAGE Sus scrofa
2.7.7.3 monomer 1 * 62000, gel filtration Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
2.7.1.24 DPCK
-
 Homo sapiens
2.7.1.24 More bifunctional enzyme is termed CoA synthase Homo sapiens
2.7.1.24 More bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 Homo sapiens
2.7.1.24 More bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.24 25
-
 assay at Homo sapiens
2.7.1.24 25
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.24 8
-
 assay at Homo sapiens
2.7.1.24 8
-
 assay at Sus scrofa