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Literature summary extracted from

  • Worrall, D.M.; Tubbs, P.K.
    A bifunctional enzyme complex in coenzyme A biosynthesis: purification of pantetheine phosphate adenylyltransferase and dephospho-CoA kinase (1983), Biochem. J., 215, 153-157.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.24 cytosol
-
 Sus scrofa 5829
-
2.7.7.3 cytosol
-
 Rattus norvegicus 5829
-
2.7.7.3 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.24 57000
-
 2 * 57000, SDS-PAGE Sus scrofa
2.7.1.24 115000
-
 gel filtration Sus scrofa
2.7.7.3 57000
-
 alpha2, 2 * 57000, SDS-PAGE Sus scrofa
2.7.7.3 115000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate Rattus norvegicus
-
 diphosphate + 3'-dephospho-CoA
-
 ?
2.7.7.3 ATP + pantetheine 4'-phosphate Sus scrofa
-
 diphosphate + 3'-dephospho-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.24 Sus scrofa
-
-
-
2.7.7.3 Rattus norvegicus
-
-
-
2.7.7.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.24
-
 Sus scrofa
2.7.7.3
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.24 liver
-
 Sus scrofa
-
2.7.7.3 liver
-
 Rattus norvegicus
-
2.7.7.3 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.24 3.68
-
-
 Sus scrofa
2.7.7.3 7.61
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.24 -20°C, 0.5 mg protein/ml, at least 1 month Sus scrofa
2.7.7.3 -20°C, 0.5 mg protein/ml, at least 1 month Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.24 ATP + dephospho-CoA
-
 Sus scrofa ADP + CoA
-
 ?
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Rattus norvegicus diphosphate + 3'-dephospho-CoA
-
 ?
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Sus scrofa diphosphate + 3'-dephospho-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.24 dimer 2 * 57000, SDS-PAGE Sus scrofa
2.7.7.3 dimer alpha2, 2 * 57000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
2.7.1.24 More bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 Sus scrofa
2.7.1.24 More bifunctional enzyme is termed CoA-synthetase Sus scrofa
2.7.7.3 More with EC 2.7.1.24 part of a bifunctional enzyme with EC 2.7.1.24 Rattus norvegicus
2.7.7.3 More with EC 2.7.1.24 part of a bifunctional enzyme with EC 2.7.1.24 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.24 30
-
 assay at Sus scrofa
2.7.7.3 30
-
 assay at Rattus norvegicus
2.7.7.3 30
-
 assay at Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.24 Sus scrofa
-
-
 5.75
2.7.7.3 Sus scrofa chromatofocusing technique with a PBE 94 column and Polybuffer 74
-
 5.75