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Literature summary extracted from

  • Rudi, H.; Doan, D.N.P.; Olsen, O.A.
    A (His)6-tagged recombinant barley (Hordeum vulgare L.) endosperm ADP-glucose pyrophosphorylase expressed in the baculovirus-insect cell system is insensitive to allosteric regulation by 3-phosphoglycerate and inorganic phosphate (1997), FEBS Lett., 419, 124-130.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate recombinant enzyme is insensitive to 3-phosphoglycerate Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 cloning of two isoforms, expressed in the baculovirus-insect cell system Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate recombinant enzyme is insensitive to phosphate Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 54000
-
 x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare
2.7.7.27 55000
-
 x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 purification of recombinant enzyme after attachment of a histidine tag on only one of the subunits of a multimeric enzyme Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Hordeum vulgare
-
2.7.7.27 seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 28.6
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 heterotetramer x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare