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Literature summary extracted from

  • Ribéreau-Gayon, G.; Sabraw, A.; Lammel, C.; Preiss, J.
    Biosynthesis of bacterial glycogen IX: regulatory properties of the adenosine diphosphate glucose pyrophosphrylases of the Enterobacterieae (1971), Arch. Biochem. Biophys., 142, 675-692.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Enterobacter cloacae
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Klebsiella aerogenes
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Escherichia aurescens
2.7.7.27 D-fructose 1,6-bisphosphate in presence of fructose 1,6-diphosphate pH-optimum decreased, effects on Km Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 D-fructose 1,6-bisphosphate in presence of fructose 1,6-diphosphate pH-optimum decreased, effects on Km Citrobacter freundii
2.7.7.27 NADPH
-
 Citrobacter freundii
2.7.7.27 NADPH activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 NADPH activation Enterobacter cloacae
2.7.7.27 NADPH activation Klebsiella aerogenes
2.7.7.27 NADPH activation Escherichia aurescens
2.7.7.27 pyridoxal 5'-phosphate activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 pyridoxal 5'-phosphate activation Enterobacter cloacae
2.7.7.27 pyridoxal 5'-phosphate activation Klebsiella aerogenes
2.7.7.27 pyridoxal 5'-phosphate activation Citrobacter freundii
2.7.7.27 pyridoxal 5'-phosphate activation Escherichia aurescens
2.7.7.27 pyridoxal 5'-phosphate influence on Km and pH-optimum Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 pyridoxal 5'-phosphate influence on Km and pH-optimum Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP inhibition under activated and unactivated conditions Citrobacter freundii
2.7.7.27 ADP
-
 Enterobacter cloacae
2.7.7.27 ADP
-
 Escherichia aurescens
2.7.7.27 ADP
-
 Klebsiella aerogenes
2.7.7.27 ADP inhibition under activated and unactivated conditions Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 AMP the most potent inhibitor, inhibition under activated and unactivated conditions Citrobacter freundii
2.7.7.27 AMP
-
 Enterobacter cloacae
2.7.7.27 AMP
-
 Escherichia aurescens
2.7.7.27 AMP
-
 Klebsiella aerogenes
2.7.7.27 AMP the most potent inhibitor, inhibition under activated and unactivated conditions Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 phosphate inhibition under activated and unactivated conditions Citrobacter freundii
2.7.7.27 phosphate
-
 Enterobacter cloacae
2.7.7.27 phosphate
-
 Escherichia aurescens
2.7.7.27 phosphate
-
 Klebsiella aerogenes
2.7.7.27 phosphate inhibition under activated and unactivated conditions Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.13
-
 alpha-D-glucose 1-phosphate pH 8.4, 37°C, influence of activators on Km Citrobacter freundii
2.7.7.27 0.21
-
 alpha-D-glucose 1-phosphate pH 8.4, 37°C, influence of activators on Km Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.72
-
 ATP pH 8.4, 37°C, influence of activators on Km Citrobacter freundii
2.7.7.27 2.4
-
 ATP pH 8.4, 37°C, influence of activators on Km Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Citrobacter freundii
-
-
-
2.7.7.27 Enterobacter cloacae
-
-
-
2.7.7.27 Escherichia aurescens
-
-
-
2.7.7.27 Klebsiella aerogenes
-
-
-
2.7.7.27 no activity in Erwinia carotovora
-
-
-
2.7.7.27 no activity in Proteus vulgaris or Erwinia carotovora
-
-
-
2.7.7.27 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 partial Citrobacter freundii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 0.45
-
-
 Citrobacter freundii
2.7.7.27 0.96
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Enterobacter cloacae diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Klebsiella aerogenes diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Citrobacter freundii diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Escherichia aurescens diphosphate + ADP-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 additional information
-
 pH optimums in various buffer and in presence and absence of fructose-1,6-bisphosphate Citrobacter freundii
2.7.7.27 7.5 8.5 broad, Tris buffer Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 8
-
 bicine buffer, HEPES buffer Citrobacter freundii
2.7.7.27 8.5
-
 Tris buffer Citrobacter freundii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 additional information
-
 additional information Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 additional information
-
 additional information Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators Citrobacter freundii