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Literature summary extracted from

  • Lehmann, M.; Preiss, J.
    Biosynthesis of bacterial glycogen: purification and properties of Salmonella typhimurium LT-2 adenosine diphosphate glucose pyrophosphorylase (1980), J. Bacteriol., 143, 120-127.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 2,3-diphosphoglycerate activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 2,3-diphosphoglycerate allosteric activator Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 2-phospho-D-glycerate activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 2-phospho-D-glycerate slight activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 3-phosphoglycerate no activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 6-phosphogluconate slight activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 D-fructose 1,6-bisphosphate allosteric activator Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 D-fructose 1,6-bisphosphate physiological modulator Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 D-Glucose 1,6-bisphosphate slight activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 D-ribose 5-phosphate no activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 glyceraldehyde 3-phosphate slight activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 NADP+ slight activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 NADPH activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 pyridoxal 5'-phosphate activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 pyridoxal 5'-phosphate allosteric activator Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
2.7.7.27 glycerol, 20% stabilizes Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP allosteric inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 AMP allosteric inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 2
-
 ATP pH 8.0, 37°C, strain JP102, kinetic study Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 2.4
-
 ATP pH 8.0, 37°C, strain LT-2, kinetic study Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
 amino acid composition and comparison Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 48000
-
 4 * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 190000
-
 sucrose density gradient centrifugation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 195000
-
 PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2 mutant JP102
-
2.7.7.27 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strains LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 106
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 -70°C, in 50 mM Tris-HCl buffer, pH 7.2, 10 mM potassium phosphate, 0.5 mM dithioerythritol, 20% glycerol, stable Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer 4 * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.028
-
 AMP pH 8.0, 37°C, strain LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.032
-
 AMP pH 8.0, 37°C, strain JP102, in presence of 0.25 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.096
-
 AMP pH 8.0, 37°C, strain JP102, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.11
-
 AMP pH 8.0, 37°C, strain LT-2, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.42
-
 ADP pH 8.0, 37°C, strains JP102 and LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 0.93
-
 phosphate pH 8.0, 37°C, strains LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 1
-
 ADP pH 8.0, 37°C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 1.1
-
 ADP pH 8.0, 37°C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 1.3
-
 phosphate pH 8.0, 37°C, strains JP102, in presence of 0.25 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 1.7
-
 phosphate pH 8.0, 37°C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.27 2
-
 phosphate pH 8.0, 37°C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate Salmonella enterica subsp. enterica serovar Typhimurium